ArticleBMC developmental biology2004
Hedgehog can drive terminal differentiation of amniote slow skeletal muscle.
Article in BMC developmental biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Prenatal X-ray exposure and rhabdomyosarcoma in children: a report from the children's oncology group.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2009Trial
- Cilia, Centrosomes and Skeletal Muscle.International journal of molecular sciences · 2021Review
- "Muscling" Throughout Life: Integrating Studies of Muscle Development, Homeostasis, and Disease in Zebrafish.Current topics in developmental biology · 2017Review
- Primary cilia control hedgehog signaling during muscle differentiation and are deregulated in rhabdomyosarcoma.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Hedgehog signaling regulates MyoD expression and activity.The Journal of biological chemistry · 2013Article
- Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity.Genes & development · 2012Article
- Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation.Genes & development · 2012Article
- Cooperation between Shh and IGF-I in promoting myogenic proliferation and differentiation via the MAPK/ERK and PI3K/Akt pathways requires Smo activity.Journal of cellular physiology · 2012Article
- Anomalous extraocular muscles with strabismus.AJNR. American journal of neuroradiology · 2011Article
- Sox6, jack of all trades: a versatile regulatory protein in vertebrate development.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Review
- Cdkn1c drives muscle differentiation through a positive feedback loop with Myod.Developmental biology · 2011Article
- Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro.PloS one · 2010Article
- N-cadherin ligation, but not Sonic hedgehog binding, initiates Cdo-dependent p38alpha/beta MAPK signaling in skeletal myoblasts.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Transgenic zebrafish line with over-expression of Hedgehog on the skin: a useful tool to screen Hedgehog-inhibiting compounds.Transgenic research · 2009Article
- Wnt signaling promotes AChR aggregation at the neuromuscular synapse in collaboration with agrin.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Muscle development and obesity: Is there a relationship?Organogenesis · 2008Article
- Myogenic growth factors can decrease extraocular muscle force generation: a potential biological approach to the treatment of strabismus.Investigative ophthalmology & visual science · 2008Article
- The hedgehog regulated oncogenes Gli1 and Gli2 block myoblast differentiation by inhibiting MyoD-mediated transcriptional activation.Oncogene · 2007Article
- Signals and myogenic regulatory factors restrict pax3 and pax7 expression to dermomyotome-like tissue in zebrafish.Developmental biology · 2007Article
- Multiple upstream modules regulate zebrafish myf5 expression.BMC developmental biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSecreted Hedgehog (Hh) signalling molecules have profound influences on many developing and regenerating tissues. Yet in most vertebrate tissues it is unclear which Hh-responses are the direct result of Hh action on a particular cell type because Hhs frequently elicit secondary signals. In developing skeletal muscle, Hhs promote slow myogenesis in zebrafish and are involved in specification of medial muscle cells in amniote somites. However, the extent to which non-myogenic cells, myoblasts or differentiating myocytes are direct or indirect targets of Hh signalling is not known.
resultsWe show that Sonic hedgehog (Shh) can act directly on cultured C2 myoblasts, driving Gli1 expression, myogenin up-regulation and terminal differentiation, even in the presence of growth factors that normally prevent differentiation. Distinct myoblasts respond differently to Shh: in some slow myosin expression is increased, whereas in others Shh simply enhances terminal differentiation. Exposure of chick wing bud cells to Shh in culture increases numbers of both muscle and non-muscle cells, yet simultaneously enhances differentiation of myoblasts. The small proportion of differentiated muscle cells expressing definitive slow myosin can be doubled by Shh. Shh over-expression in chick limb bud reduces muscle mass at early developmental stages while inducing ectopic slow muscle fibre formation. Abundant later-differentiating fibres, however, do not express extra slow myosin. Conversely, Hh loss of function in the limb bud, caused by implanting hybridoma cells expressing a functionally blocking anti-Hh antibody, reduces early slow muscle formation and differentiation, but does not prevent later slow myogenesis. Analysis of Hh knockout mice indicates that Shh promotes early somitic slow myogenesis.
conclusionsTaken together, the data show that Hh can have direct pro-differentiative effects on myoblasts and that early-developing muscle requires Hh for normal differentiation and slow myosin expression. We propose a simple model of how direct and indirect effects of Hh regulate early limb myogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.