Evidence map›Paper›PMID 15235813›Full record

ArticlePlant cell reports2004

Transgene expression in strawberries driven by a heterologous phloem-specific promoter.

Yan Zhao, Qingzhong Liu, Robert E Davis

Abstract read
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In one paragraph

Article in Plant cell reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Review
  2. Article
  3. An EfficientPlants (Basel, Switzerland) · 2024
    Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. PromoterFrontiers in genetics · 2020
    Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yan ZhaoMolecular Plant Pathology Laboratory, USDA-Agriculture Research Service, Beltsville, MD 20705, USA. zhaoy@ba.ars.usda.gov
Qingzhong Liu
Robert E Davis

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Strawberry is susceptible to diseases caused by phytoplasmas, mycoplasma-like prokaryotes restricted to sieve elements in the phloem tissue of infected plants. One strategy to improve strawberry resistance to phytoplasmas involves transgenic expression of anti-microbial peptide genes in phloem. For targeted phloem-specific expression, we constructed a binary vector with an expression cassette bearing the beta-glucuronidase (GUS) reporter gene (uidA) under control of the Arabidopsis sucrose-H+ symporter gene (AtSUC2) promoter. Transgenic strawberry lines were generated with high efficiencies by a modified transformation protocol, which combines the adoption of a 3-day pre-selection period following transformation, and the addition of 10-microM thidiazuron to the regeneration medium. Histological GUS activity indicated that the reporter gene was expressed specifically in phloem of leaves, petioles, and roots of transgenic plants. The results suggest that the transformation protocol and the AtSUC2 promoter may be useful for engineering phytoplasma-resistant transgenic strawberries.

Indexed as

ArabidopsisFragariaGene Expression Regulation, PlantGenes, ReporterGenetic EngineeringGenetic VectorsGlucuronidaseImmunity, InnateMembrane Transport ProteinsPhenylurea CompoundsPlant LeavesPlant ProteinsPlant RootsPlants, Genetically ModifiedPromoter Regions, GeneticThiadiazolesGlucuronidaseMembrane Transport ProteinsPhenylurea CompoundsPlant Proteinssucrose transport protein, plantThiadiazolesthidiazuron

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.