ArticleThe Journal of physiology2004
Role of interstitial cells and gap junctions in the transmission of spontaneous Ca2+ signals in detrusor smooth muscles of the guinea-pig urinary bladder.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed.
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Defining the molecular fingerprint of bladder and kidney fibroblasts.American journal of physiology. Renal physiology · 2023Review
- Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRAAmerican journal of physiology. Renal physiology · 2022Article
- The effect of amino acids on the bladder cycle: a concise review.Amino acids · 2022Review
- Propagation of Pacemaker Activity and Peristaltic Contractions in the Mouse Renal Pelvis Rely on CaFunction (Oxford, England) · 2022Article
- The Dependence of Urinary Bladder Responses on Extracellular Calcium Varies Between Muscarinic, Histamine, 5-HT (Serotonin), Neurokinin, Prostaglandin, and Angiotensin Receptor Activation.Frontiers in physiology · 2022Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Spontaneous synaptic drive in detrusor smooth muscle: computational investigation and implications for urinary bladder function.Journal of computational neuroscience · 2019Article
- ATP transients accompany spontaneous contractions in isolated guinea-pig detrusor smooth muscle.Experimental physiology · 2019Article
- Smooth muscle gap-junctions allow propagation of intercellular CaCell calcium · 2018Article
- What are the origins and relevance of spontaneous bladder contractions? ICI-RS 2017.Neurourology and urodynamics · 2018Review
- Comparative immunohistochemical characterization of interstitial cells in the urinary bladder of human, guinea pig and pig.Histochemistry and cell biology · 2018Article
- Investigation of the Syncytial Nature of Detrusor Smooth Muscle as a Determinant of Action Potential Shape.Frontiers in physiology · 2018Article
- Modulation of Bladder Wall Micromotions Alters Intravesical Pressure Activity in the Isolated Bladder.Frontiers in physiology · 2018Article
- Acute radiation impacts contractility of guinea-pig bladder strips affecting mucosal-detrusor interactions.PloS one · 2018Article
- The Dome Wall of Bladder Acts as a Pacemaker Site in Detrusor Instability in Rats.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Calcium Signaling in Interstitial Cells: Focus on Telocytes.International journal of molecular sciences · 2017Review
- Proliferation of Interstitial Cells in the Cyclophosphamide-Induced Cystitis and the Preventive Effect of Imatinib.BioMed research international · 2017Article
- The Effect of Gap Junctional Coupling on the Spatiotemporal Patterns of Ca2+ Signals and the Harmonization of Ca2+-Related Cellular Responses.PLoS computational biology · 2016Article
- The actions of prolonged exposure to cholinergic agonists on isolated bladder strips from the rat.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate mechanisms underlying the transmission of spontaneous Ca2+ signals in the bladder, changes in intracellular concentrations of Ca2+ ([Ca2+]i) were visualized in isolated detrusor smooth muscle bundles of the guinea-pig urinary bladder loaded with a fluorescent Ca2+ indicator, fura-PE3 or fluo-4. Spontaneous increases in [Ca2+]i (Ca2+ transients) preferentially originated along the boundary of muscle bundles and then spread to the other boundary (Ca2+ waves). The synchronicity of Ca2+ waves across the bundles was disrupted by 18beta-glycyrrhetinic acid (18beta-GA, 40 microm), carbenoxolone (30 microm) or 2-aminoethoxydiphenylborate (2-APB, 50-100 microm), while CPA (10 microm), ryanodine (100 microm), xestospongin C (3 microm) and U-73122 (10 microm) had no effect. Intracellular recordings using two independent microelectrodes demonstrated that 2-APB (100 microm) blocked electrical coupling between detrusor smooth muscle cells. Nifedipine (10 microm) but not nominal Ca2+-free solution diminished the synchronicity of Ca2+ waves before preventing their generation. Staining for c-kit identified interstitial cells (IC) located along both boundaries of muscle bundles. IC were also scattered amongst smooth muscle cells and were more dominantly distributed in connective tissue between muscle bundles. IC generated nifedipine-resistant spontaneous Ca2+ transients, which occurred independently of those of smooth muscles. In conclusion, the propagation of Ca2+ transients in the bladder appears to be exclusively mediated by the spread of action potentials through gap junctions being facilitated by the regenerative nature of L-type Ca2+ channels, without significant contribution of intracellular Ca2+ stores. IC in the bladder may modulate the transmission of Ca2+ transients originating from smooth muscle cells rather than being the pacemaker of spontaneous activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.