ArticleThe Journal of physiology2004
Preferential accumulation of GABAA receptor gamma 2L, not gamma 2S, cytoplasmic loops at rat spinal cord inhibitory synapses.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
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- Electroacupuncture at PC6 (Neiguan) Attenuates Angina Pectoris in Rats with Myocardial Ischemia-Reperfusion Injury Through Regulating the Alternative Splicing of the Major Inhibitory Neurotransmitter Receptor GABRG2.Journal of cardiovascular translational research · 2022Article
- A comprehensive atlas of fetal splicing patterns in the brain of adult myotonic dystrophy type 1 patients.NAR genomics and bioinformatics · 2022Article
- Adaptive Balance in Posterior Cerebellum.Frontiers in neurology · 2021Review
- Genome-Wide Analysis of Differential Gene Expression and Splicing in Excitatory Neurons and Interneuron Subtypes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- γ2 GABAFrontiers in cellular neuroscience · 2018Review
- γ1-Containing GABA-A Receptors Cluster at Synapses Where they Mediate Slower Synaptic Currents than γ2-Containing GABA-A Receptors.Frontiers in molecular neuroscience · 2017Article
- Chloride transporter KCC2-dependent neuroprotection depends on the N-terminal protein domain.Cell death & disease · 2015Article
- Identification of a new genomic hot spot of evolutionary diversification of protein function.PloS one · 2015Article
- Long-term climbing fibre activity induces transcription of microRNAs in cerebellar Purkinje cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Climbing fiber activity reduces 14-3-3-θ regulated GABA(A) receptor phosphorylation in cerebellar Purkinje cells.Neuroscience · 2012Article
- Gephyrin, the enigmatic organizer at GABAergic synapses.Frontiers in cellular neuroscience · 2012Article
- Inhibition to excitation ratio regulates visual system responses and behavior in vivo.Journal of neurophysiology · 2011Article
- Climbing fiber-evoked Purkinje cell discharge reduces expression of GABA(A) receptor-associated protein and decreases its interaction with GABA(A) receptors.Journal of neurochemistry · 2011Article
- Climbing fibers induce microRNA transcription in cerebellar Purkinje cells.Neuroscience · 2010Article
- Complex role of collybistin and gephyrin in GABAA receptor clustering.The Journal of biological chemistry · 2010Article
- Alcoholism and alternative splicing of candidate genes.International journal of environmental research and public health · 2010Review
- Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid(A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update.Pharmacological reviews · 2008Review
- GABAA receptors are expressed and facilitate relaxation in airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing generates two variants of the GABAAR gamma2-subunit, gamma2S and gamma2L, which differ by insertion of the amino acid sequence LLRMFSFK into the large cytoplasmic loop between transmembrane domains 3 and 4. This additional sequence within the GABAAR gamma2L-subunit contains the potential protein kinase C (PKC) phosphorylation site serine 343 (Ser343). In the present study we intended to determine the capacity of these two splice variants to accumulate at inhibitory synaptic terminals and to colocalize with gephyrin, and to find out whether phosphorylation of Ser343 has any effect on GABAAR distribution. Green fluorescent protein (GFP)-tagged large cytoplasmic loops of GABAAR gamma2S and gamma2L (GFP::gamma2S/L) were used as surrogates for full-length receptors to study the function of the individual gamma2S and gamma2L peptides in transfected spinal cord neurones (SCNs) and COS-7 cells. It was found that GFP::gamma2L displayed a significantly higher capacity to accumulate at inhibitory synapses than GFP::gamma2S. GABAAR GFP::gamma2S accumulation at inhibitory postsynaptic sites was suppressed to the extent that GFP::gamma2S assumed a diffuse cytosolic distribution. PKC activation facilitated the postsynaptic clustering of GFP::gamma2L but not of GFP::gamma2S. This required the Ser343 residue, since substituting Ala343 for Ser343 produced a diffuse cytosolic localization pattern, like that of GFP::gamma2S. Furthermore, upon PKC activation Discosoma Red2-tagged GABAAR gamma2L (DsRed 2::gamma2L) colocalized with gephyrin in transfected COS-7 cells. These results support the idea that alternative splicing regulates the access of GABAARs to inhibitory postsynaptic sites in a Ser343 phosphorylation-regulated way.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.