ArticleJournal of bacteriology2004
Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Overview of Phage Defense Systems in Bacteria and Their Applications.International journal of molecular sciences · 2024Review
- Characterization of the Attachment of Three New Coliphages onto the Ferrichrome Transporter FhuA.Journal of virology · 2023Article
- Deciphering Bacteriophage T5 Host Recognition Mechanism and Infection Trigger.Journal of virology · 2023Article
- Comparative Genomics of a PolyvalentViruses · 2023Article
- Structural basis for host recognition and superinfection exclusion by bacteriophage T5.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.Chemical reviews · 2022Review
- Systematic strategies for developing phage resistant Escherichia coli strains.Nature communications · 2022Article
- The Biology of Colicin M and Its Orthologs.Antibiotics (Basel, Switzerland) · 2021Review
- Native Cell Environment Constrains Loop Structure in the Escherichia coli Cobalamin Transporter BtuB.Biophysical journal · 2020Article
- Exploiting phage receptor binding proteins to enable endolysins to kill Gram-negative bacteria.Scientific reports · 2020Article
- The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model.Nature communications · 2019Article
- Screening of Polyvalent Phage-ResistantFrontiers in microbiology · 2019Article
- Evolution and Sequence Diversity of FhuA in Salmonella and Escherichia.Infection and immunity · 2018Article
- Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water.PloS one · 2018Article
- Bacteriophage lambda: Early pioneer and still relevant.Virology · 2015Review
- Bacteriophage as instructional organisms in introductory biology labs.Bacteriophage · 2014Article
- Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Genome of Enterobacteriophage Lula/phi80 and insights into its ability to spread in the laboratory environment.Journal of bacteriology · 2012Article
- Redesign of a plugged beta-barrel membrane protein.The Journal of biological chemistry · 2011Article
- Coupling of calcium and substrate binding through loop alignment in the outer-membrane transporter BtuB.Journal of molecular biology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise deletions of cell surface-exposed loops of FhuA resulted in mutants of Escherichia coli with distinct phenotypes. Deletion of loop 3 or 11 inactivated ferrichrome transport activity. Deletion of loop 8 inactivated receptor activity for colicin M and the phages T1, T5, and phi80. The loop 7 deletion mutant was colicin M resistant but fully phage sensitive. The loop 4 deletion mutant was resistant to the TonB-dependent phages T1 and phi80 but fully sensitive to the TonB-independent phage T5. The phenotypes of the deletion mutants revealed important sites for the multiple FhuA transport and receptor activities. The ligand binding sites are nonidentical and are distributed among the entire exposed surface. Presumably, FhuA evolved as a ferrichrome transporter and was subsequently used as a receptor by the phages and colicin M, which selected the same as well as distinct loops as receptor sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.