ArticleJournal of bacteriology2004
The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
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- Metabolic Engineering ofMicroorganisms · 2023Review
- Construction of Glucose-6-Phosphate Dehydrogenase Overexpression Strain ofMarine drugs · 2022Article
- Constitutive glucose dehydrogenase elevates intracellular NADPH levels and luciferase luminescence in Bacillus subtilis.Microbial cell factories · 2022Article
- Species interactions constrain adaptation and preserve ecological stability in an experimental microbial community.The ISME journal · 2022Article
- Physiological and transcriptomic analyses to reveal underlying phenolic acid action in consecutive monoculture problem of Polygonatum odoratum.BMC plant biology · 2021Article
- The Natural AntimicrobialFoods (Basel, Switzerland) · 2021Article
- Impact of activation of neotrehalosadiamine/kanosamine biosynthetic pathway on the metabolism ofJournal of bacteriology · 2021Article
- Identification of Key Metabolites in Poly-γ-Glutamic Acid Production by Tuning γ-PGA Synthetase Expression.Frontiers in bioengineering and biotechnology · 2020Article
- Enhanced Biosynthesis of 2-Deoxy-Frontiers in microbiology · 2018Article
- In silico model-driven cofactor engineering strategies for improving the overall NADP(H) turnover in microbial cell factories.Journal of industrial microbiology & biotechnology · 2015Article
- The molecular timeline of a reviving bacterial spore.Molecular cell · 2015Article
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- Sigma S-dependent antioxidant defense protects stationary-phase Escherichia coli against the bactericidal antibiotic gentamicin.Antimicrobial agents and chemotherapy · 2014Article
- Enhancement of L-ornithine production by disruption of three genes encoding putative oxidoreductases in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2014Article
- The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Implication of gluconate kinase activity in L-ornithine biosynthesis in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2012Article
- 13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis.The Journal of biological chemistry · 2012Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the importance of the oxidative pentose phosphate (PP) pathway as a major source of reducing power and metabolic intermediates for biosynthetic processes, almost no direct genetic or biochemical evidence is available for Bacillus subtilis. Using a combination of knockout mutations in known and putative genes of the oxidative PP pathway and 13C-labeling experiments, we demonstrated that yqjI encodes the NADP+-dependent 6-P-gluconate dehydrogenase, as was hypothesized previously from sequence similarities. Moreover, YqjI was the predominant isoenzyme during glucose and gluconate catabolism, and its role in the oxidative PP pathway could not be played by either of two homologues, GntZ and YqeC. This conclusion is in contrast to the generally held view that GntZ is the relevant isoform; hence, we propose a new designation for yqjI, gndA, the monocistronic gene encoding the principal 6-P-gluconate dehydrogenase. Although we demonstrated the NAD+-dependent 6-P-gluconate dehydrogenase activity of GntZ, gntZ mutants exhibited no detectable phenotype on glucose, and GntZ did not contribute to PP pathway fluxes during growth on glucose. Since gntZ mutants grew normally on gluconate, the functional role of GntZ remains obscure, as does the role of the third homologue, YqeC. Knockout of the glucose-6-P dehydrogenase-encoding zwf gene was primarily compensated for by increased glycolytic fluxes, but about 5% of the catabolic flux was rerouted through the gluconate bypass with glucose dehydrogenase as the key enzyme.
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