ArticleJournal of bacteriology2004
Phosphorus limitation enhances biofilm formation of the plant pathogen Agrobacterium tumefaciens through the PhoR-PhoB regulatory system.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed.
- Gnotobiotic growth and phosphorus limitation of Arabidopsis thaliana and co-occurring microbes on phosphated iron oxides.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- Small DUF1127 proteins regulate bacterial phosphate metabolism through protein-protein interactions with the sensor kinase PhoR.microLife · 2025Article
- The effect of NaCl, pH, and phosphate on biofilm formation and exopolysaccharide production by high biofilm producers of Bacillus strains.Folia microbiologica · 2024Article
- An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria.Microbiology resource announcements · 2023Article
- The effects of temperature, salt, and phosphate on biofilm and exopolysaccharide production by Azotobacter spp.Archives of microbiology · 2023Article
- Integrating phylogenetic and functional data in microbiome studies.Bioinformatics (Oxford, England) · 2022Article
- Conformation and dynamic interactions of the multipartite genome inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Coordination of Phosphate and Magnesium Metabolism in Bacteria.Advances in experimental medicine and biology · 2022Article
- Article
- Arginine-Rich Small Proteins with a Domain of Unknown Function, DUF1127, Play a Role in Phosphate and Carbon Metabolism of Agrobacterium tumefaciens.Journal of bacteriology · 2020Article
- Short, Rich, and Powerful: a New Family of Arginine-Rich Small Proteins Have Outsized Impact in Agrobacterium tumefaciens.Journal of bacteriology · 2020Article
- Regulation ofJournal of bacteriology · 2019Article
- Phosphate deficiency induced biofilm formation of Burkholderia on insoluble phosphate granules plays a pivotal role for maximum release of soluble phosphate.Scientific reports · 2019Article
- Bacterial adhesion at the single-cell level.Nature reviews. Microbiology · 2018Review
- Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Ecological and evolutionary dynamics of a model facultative pathogen: Agrobacterium and crown gall disease of plants.Environmental microbiology · 2018Review
- A Rhizobiales-Specific Unipolar Polysaccharide Adhesin Contributes to Rhodopseudomonas palustris Biofilm Formation across Diverse Photoheterotrophic Conditions.Applied and environmental microbiology · 2017Article
- Igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with Rhizoglomus irregulare DAOM 197198.Mycorrhiza · 2017Article
- Global Dynamic Proteome Study of a Pellicle-forming Acinetobacter baumannii Strain.Molecular & cellular proteomics : MCP · 2017Article
- The stress-related, rhizobial small RNA RcsR1 destabilizes the autoinducer synthase encoding mRNA sinI in Sinorhizobium meliloti.RNA biology · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant pathogen Agrobacterium tumefaciens forms architecturally complex biofilms on inert surfaces. Adherence of A. tumefaciens C58 was significantly enhanced under phosphate limitation compared to phosphate-replete conditions, despite slower overall growth under low-phosphate conditions. Replacement of Pi with sn-glycerol-3-phosphate and 2-aminoethylphosphonate yielded similar results. The increase in surface interactions under phosphate limitation was observed in both static culture and continuous-culture flow cells. Statistical analysis of confocal micrographs obtained from the flow cell biofilms revealed that phosphate limitation increased both the overall attached biomass and the surface coverage, whereas the maximum thickness of the biofilm was not affected. Functions encoded on the two large plasmids of A. tumefaciens C58, pTiC58 and pAtC58, were not required for the observed phosphate effect. The phosphate concentration at which increased attachment was observed triggered the phosphate limitation response, controlled in many bacteria by the two-component regulatory system PhoR-PhoB. The A. tumefaciens phoB and phoR orthologues could only be disrupted in the presence of plasmid-borne copies of the genes, suggesting that this regulatory system might be essential. Expression of the A. tumefaciens phoB gene from a tightly regulated inducible promoter, however, correlated with the amount of biofilm under both phosphate-limiting and nonlimiting conditions, demonstrating that components of the Pho regulon influence A. tumefaciens surface interactions.
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