ArticleJournal of nanobiotechnology2004
Modification of a neuronal network direction using stepwise photo-thermal etching of an agarose architecture.
Article in Journal of nanobiotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- An integratediScience · 2026Article
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Thermoplasmonic neural chip platform for in situ manipulation of neuronal connections in vitro.Nature communications · 2020Article
- Dominant rule of community effect in synchronized beating behavior of cardiomyocyte networks.Biophysical reviews · 2020Review
- Live-Cell, Label-Free Identification of GABAergic and Non-GABAergic Neurons in Primary Cortical Cultures Using Micropatterned Surface.PloS one · 2016Article
- Isolation, culture, and induced multiple differentiation of Mongolian sheep bone marrow-derived mesenchymal stem cells.In vitro cellular & developmental biology. Animal · 2014Article
- Microtechnologies to fuel neurobiological research with nanometer precision.Journal of nanobiotechnology · 2013Review
- On-chip cellomics assay enabling algebraic and geometric understanding of epigenetic information in cellular networks of living systems. 1. Temporal aspects of epigenetic information in bacteria.Sensors (Basel, Switzerland) · 2012Review
- On-chip constructive cell-network study (I): contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect.Journal of nanobiotechnology · 2011Article
- Biochip∕laser cell deposition system to assess polarized axonal growth from single neurons and neuron∕glia pairs in microchannels with novel asymmetrical geometries.Biomicrofluidics · 2011Article
- Phagocytic response to fully controlled plural stimulation of antigens on macrophage using on-chip microcultivation system.Journal of nanobiotechnology · 2006Article
- Stability of beating frequency in cardiac myocytes by their community effect measured by agarose microchamber chip.Journal of nanobiotechnology · 2005Article
- A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization.Journal of nanobiotechnology · 2004Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Control over spatial distribution of individual neurons and the pattern of neural network provides an important tool for studying information processing pathways during neural network formation. Moreover, the knowledge of the direction of synaptic connections between cells in each neural network can provide detailed information on the relationship between the forward and feedback signaling. We have developed a method for topographical control of the direction of synaptic connections within a living neuronal network using a new type of individual-cell-based on-chip cell-cultivation system with an agarose microchamber array (AMCA). The advantages of this system include the possibility to control positions and number of cultured cells as well as flexible control of the direction of elongation of axons through stepwise melting of narrow grooves. Such micrometer-order microchannels are obtained by photo-thermal etching of agarose where a portion of the gel is melted with a 1064-nm infrared laser beam. Using this system, we created neural network from individual Rat hippocampal cells. We were able to control elongation of individual axons during cultivation (from cells contained within the AMCA) by non-destructive stepwise photo-thermal etching. We have demonstrated the potential of our on-chip AMCA cell cultivation system for the controlled development of individual cell-based neural networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.