ArticleNucleic acids research2004
Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Deep learning-based investigation of chloroplast translation regulatory sequences.Frontiers in plant science · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.ACS synthetic biology · 2024Article
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology.Plant physiology · 2019Article
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
- Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco.Journal of experimental botany · 2017Article
- Translation initiation factor 3 families: what are their roles in regulating cyanobacterial and chloroplast gene expression?Photosynthesis research · 2015Review
- Processing of the 5'-UTR and existence of protein factors that regulate translation of tobacco chloroplast psbN mRNA.Plant molecular biology · 2014Article
- The translational apparatus of plastids and its role in plant development.Molecular plant · 2014Review
- RBF1, a plant homolog of the bacterial ribosome-binding factor RbfA, acts in processing of the chloroplast 16S ribosomal RNA.Plant physiology · 2014Article
- Additional pathway to translate the downstream ndhK cistron in partially overlapping ndhC-ndhK mRNAs in chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- The downstream atpE cistron is efficiently translated via its own cis-element in partially overlapping atpB-atpE dicistronic mRNAs in chloroplasts.Nucleic acids research · 2011Article
- Expression of plastid genes: organelle-specific elaborations on a prokaryotic scaffold.Plant physiology · 2011Article
- Selection of Shine-Dalgarno sequences in plastids.Nucleic acids research · 2011Article
- ATP synthase repression in tobacco restricts photosynthetic electron transport, CO2 assimilation, and plant growth by overacidification of the thylakoid lumen.The Plant cell · 2011Article
- Implications of high level pseudogene transcription in Mycobacterium leprae.BMC genomics · 2009Article
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2 authors.
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Abstract
The mechanism of translational initiation differs between prokaryotes and eukaryotes. Prokaryotic mRNAs generally contain within their 5'-untranslated region (5'-UTR) a Shine-Dalgarno (SD) sequence that serves as a ribosome-binding site. Chloroplasts possess prokaryotic-like translation machinery, and many chloroplast mRNAs have an SD-like sequence, but its position is variable. Tobacco chloroplast atpB mRNAs contain no SD-like sequence and are U-rich in the 5'-UTR (-20 to -1 with respect to the start codon). In vitro translation assays with mutated mRNAs revealed that an unstructured sequence encompassing the start codon, the AUG codon and its context are required for translation. UV crosslinking experiments showed that a 50 kDa protein (p50) binds to the 5'-UTR. Insertion of an additional initiation region (SD-sequence and AUG) in the 5'-UTR, but not downstream, arrested translation from the authentic site; however, no inhibition was observed by inserting only an AUG triplet. We hypothesize for translational initiation of the atpB mRNA that the ribosome enters an upstream region, slides to the start codon and forms an initiation complex with p50 and other components.
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