ArticleMolecular and cellular biology2004
Transcriptional coactivator PC4 stimulates promoter escape and facilitates transcriptional synergy by GAL4-VP16.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Positive coactivator PC4 shows dynamic nucleolar distribution required for rDNA transcription and protein synthesis.Cell communication and signaling : CCS · 2025Article
- The Catalytic Subunit ofInternational journal of molecular sciences · 2022Article
- The Role of VP16 in the Life Cycle of Alphaherpesviruses.Frontiers in microbiology · 2020Review
- Transcriptional positive cofactor 4 promotes breast cancer proliferation and metastasis through c-Myc mediated Warburg effect.Cell communication and signaling : CCS · 2019Article
- The human positive cofactor 4 promotes androgen-independent prostate cancer development and progression through HIF-1α/β-catenin pathway.American journal of cancer research · 2019Article
- A biochemical and biophysical model of G-quadruplex DNA recognition by positive coactivator of transcription 4.The Journal of biological chemistry · 2017Article
- Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica.Scientific reports · 2016Article
- The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators.The Journal of biological chemistry · 2015Article
- Cooperation between SMYD3 and PC4 drives a distinct transcriptional program in cancer cells.Nucleic acids research · 2015Article
- Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene.PloS one · 2013Article
- Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes.Nucleic acids research · 2012Article
- Sub1 and RPA associate with RNA polymerase II at different stages of transcription.Molecular cell · 2011Article
- Role of helix-loop-helix proteins during differentiation of erythroid cells.Molecular and cellular biology · 2011Review
- Retroviral integration site selection.Viruses · 2010Article
- Heterogeneous nuclear ribonucleoprotein R enhances transcription from the naturally configured c-fos promoter in vitro.The Journal of biological chemistry · 2009Article
- Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Recruitment of RNA polymerase II cofactor PC4 to DNA damage sites.The Journal of cell biology · 2008Article
- Human U2 snRNA genes exhibit a persistently open transcriptional state and promoter disassembly at metaphase.Molecular and cellular biology · 2008Article
- Transcriptional coactivator PC4, a chromatin-associated protein, induces chromatin condensation.Molecular and cellular biology · 2006Article
- UBF activates RNA polymerase I transcription by stimulating promoter escape.The EMBO journal · 2006Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Positive cofactor 4 (PC4) is a coactivator that strongly augments transcription by various activators, presumably by facilitating the assembly of the preinitiation complex (PIC). However, our previous observation of stimulation of promoter escape in GAL4-VP16-dependent transcription in the presence of PC4 suggested a possible role for PC4 in this step. Here, we performed quantitative analyses of the stimulatory effects of PC4 on initiation, promoter escape, and elongation in GAL4-VP16-dependent transcription and found that PC4 possesses the ability to stimulate promoter escape in response to GAL4-VP16 in addition to its previously demonstrated effect on PIC assembly. This stimulatory effect of PC4 on promoter escape required TFIIA and the TATA box binding protein-associated factor subunits of TFIID. Furthermore, PC4 displayed physical interactions with both TFIIH and GAL4-VP16 through its coactivator domain, and these interactions were regulated distinctly by PC4 phosphorylation. Finally, GAL4-VP16 and PC4 stimulated both initiation and promoter escape to similar extents on the promoters with three and five GAL4 sites; however, they stimulated promoter escape preferentially on the promoter with a single GAL4 site. These results provide insight into the mechanism by which PC4 permits multiply bound GAL4-VP16 to attain synergy to achieve robust transcriptional activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.