ArticleMolecular and cellular biology2004
TATA-binding protein mutants that are lethal in the absence of the Nhp6 high-mobility-group protein.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- A lethal ORC ATPase mutation is suppressed by alterations in ORC and RNA Pol II transcription components.PloS one · 2026Article
- The HMGB chromatin protein Nhp6A can bypass obstacles when traveling on DNA.Nucleic acids research · 2020Article
- FACT and Ash1 promote long-range and bidirectional nucleosome eviction at the HO promoter.Nucleic acids research · 2020Article
- Article
- Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nucleosomes Are Essential for Proper Regulation of a Multigated Promoter in Saccharomyces cerevisiae.Genetics · 2016Article
- Spatiotemporal cascade of transcription factor binding required for promoter activation.Molecular and cellular biology · 2015Article
- Repressive chromatin affects factor binding at yeast HO (homothallic switching) promoter.The Journal of biological chemistry · 2011Article
- Histone Chaperones Spt6 and FACT: Similarities and Differences in Modes of Action at Transcribed Genes.Genetics research international · 2011Article
- The E2F functional analogue SBF recruits the Rpd3(L) HDAC, via Whi5 and Stb1, and the FACT chromatin reorganizer, to yeast G1 cyclin promoters.The EMBO journal · 2009Article
- FACT and Asf1 regulate nucleosome dynamics and coactivator binding at the HO promoter.Molecular cell · 2009Article
- Different genetic functions for the Rpd3(L) and Rpd3(S) complexes suggest competition between NuA4 and Rpd3(S).Molecular and cellular biology · 2008Article
- Regulation of the yeast Ace2 transcription factor during the cell cycle.The Journal of biological chemistry · 2008Article
- Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II.Nucleic acids research · 2008Article
- Forkhead proteins control the outcome of transcription factor binding by antiactivation.The EMBO journal · 2007Article
- Chd1 and yFACT act in opposition in regulating transcription.Molecular and cellular biology · 2007Article
- Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Opposing roles for Set2 and yFACT in regulating TBP binding at promoters.The EMBO journal · 2006Article
- SWI/SNF binding to the HO promoter requires histone acetylation and stimulates TATA-binding protein recruitment.Molecular and cellular biology · 2006Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae Nhp6 protein is related to the high-mobility-group B family of architectural DNA-binding proteins that bind DNA nonspecifically but bend DNA sharply. Nhp6 is involved in transcriptional activation by both RNA polymerase II (Pol II) and Pol III. Our previous genetic studies have implicated Nhp6 in facilitating TATA-binding protein (TBP) binding to some Pol II promoters in vivo, and we have used a novel genetic screen to isolate 32 new mutations in TBP that are viable in wild-type cells but lethal in the absence of Nhp6. The TBP mutations that are lethal in the absence of Nhp6 cluster in three regions: on the upper surface of TBP that may have a regulatory role, near residues that contact Spt3, or near residues known to contact either TFIIA or Brf1 (in TFIIIB). The latter set of mutations suggests that Nhp6 becomes essential when a TBP mutant compromises its ability to interact with either TFIIA or Brf1. Importantly, the synthetic lethality for some of the TBP mutations is suppressed by a multicopy plasmid with SNR6 or by an spt3 mutation. It has been previously shown that nhp6ab mutants are defective in expressing SNR6, a Pol III-transcribed gene encoding the U6 splicing RNA. Chromatin immunoprecipitation experiments show that TBP binding to SNR6 is reduced in an nhp6ab mutant. Nhp6 interacts with Spt16/Pob3, the yeast equivalent of the FACT elongation complex, consistent with nhp6ab cells being extremely sensitive to 6-azauracil (6-AU). However, this 6-AU sensitivity can be suppressed by multicopy SNR6 or BRF1. Additionally, strains with SNR6 promoter mutations are sensitive to 6-AU, suggesting that decreased SNR6 RNA levels contribute to 6-AU sensitivity. These results challenge the widely held belief that 6-AU sensitivity results from a defect in transcriptional elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.