ArticleThe Journal of clinical investigation1992
On the mechanism of parathyroid hormone stimulation of calcium uptake by mouse distal convoluted tubule cells.
Article in The Journal of clinical investigation, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 122 citations in OpenAlex.
- Renal Phosphate Reabsorption is Correlated with the Increase in Lumbar Bone Mineral Density in Patients Receiving Once-Weekly Teriparatide.Calcified tissue international · 2016Trial
- Navigating the multifaceted intricacies of the NaPhysiological reviews · 2024Review
- CFTR-mediated anion secretion in parathyroid hormone-treated Caco-2 cells is associated with PKA and PI3K phosphorylation but not intracellular pH changes or NaBiochemistry and biophysics reports · 2021Article
- Five-year Estimated Glomerular Filtration Rate in Patients With Hypoparathyroidism Treated With and Without rhPTH(1-84).The Journal of clinical endocrinology and metabolism · 2020Article
- Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport.The Journal of biological chemistry · 2019Article
- A case report of T-box 1 mutation causing phenotypic features of chromosome 22q11.2 deletion syndrome.Clinical diabetes and endocrinology · 2019Article
- Continuous infusion of PTHScientific reports · 2018Article
- Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells.Biochimica et biophysica acta. General subjects · 2017Article
- Vitamin D Toxicity in Young Breastfed Infants: Report of 2 Cases.Global pediatric health · 2017Article
- Skeletal complications in cancer patients with bone metastases.International journal of urology : official journal of the Japanese Urological Association · 2016Review
- Increased serum fibroblast growth factor-23 and decreased bone turnover in patients with systemic lupus erythematosus under treatment with cyclosporine and steroid but not steroid only.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2015Article
- A new model of the distal convoluted tubule.American journal of physiology. Renal physiology · 2012Article
- Parathyroid hormone (PTH) regulates the sodium chloride cotransporter via Ras guanyl releasing protein 1 (Ras-GRP1) and extracellular signal-regulated kinase (ERK)1/2 mitogen-activated protein kinase (MAPK) pathway.Translational research : the journal of laboratory and clinical medicine · 2011Article
- Identification and apical membrane localization of an electrogenic Na⁺/Ca²⁺ exchanger NCX2a likely to be involved in renal Ca²⁺ excretion by seawater fish.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
- The regulation of parathyroid hormone secretion and synthesis.Journal of the American Society of Nephrology : JASN · 2011Review
- Pin1 regulates parathyroid hormone mRNA stability.The Journal of clinical investigation · 2009Article
- Impaired renal calcium absorption in mice lacking calcium channel beta 3 subunits.Canadian journal of physiology and pharmacology · 2009Article
- Cell models for studying renal physiology.Pflugers Archiv : European journal of physiology · 2008Review
- Parathyroid hormone increases cytosolic calcium in neonatal nephron through protein kinase C pathway.Pediatric nephrology (Berlin, Germany) · 2004Article
- Functional differences between cardiac and renal isoforms of the rat Na+-Ca2+ exchanger NCX1 expressed in Xenopus oocytes.The Journal of physiology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
PTH stimulates transcellular Ca2+ absorption in renal distal convoluted tubules. The effect of PTH on membrane voltage, the ionic basis of the change in voltage, and the relations between voltage and calcium entry were determined on immortalized mouse distal convoluted tubule cells. PTH (10(-8) M) significantly increased 45Ca2+ uptake from basal levels of 2.81 +/- 0.16 to 3.88 +/- 0.19 nmol min-1 mg protein-1. PTH-induced 45Ca2+ uptake was abolished by the dihydropyridine antagonist, nifedipine (10(-5) M). PTH did not affect 22Na+ uptake. Intracellular calcium activity ([Ca2+]i) was measured in cells loaded with fura-2. Control [Ca2+]i averaged 112 +/- 21 nM. PTH increased [Ca2+]i over the range of 10(-11) to 10(-7) M. Maximal stimulation to 326 +/- 31 nM was achieved at 10(-8) M PTH. Resting membrane voltage measured with the potential sensitive dye DiO6(3) averaged -71 +/- 2 mV. PTH hyperpolarized cells by 19 +/- 4 mV. The chloride-channel blocker NPPB prevented PTH-induced hyperpolarization. PTH decreased and NPPB increased intracellular chloride, measured with the fluorescent dye SPQ. Chloride permeability was estimated by measuring the rate of 125I- efflux. PTH increased 125I- efflux and this effect was blocked by NPPB. Clamping voltage with K+/valinomycin; depolarizing membrane voltage by reducing extracellular chloride; or addition of NPPB prevented PTH-induced calcium uptake. In conclusion, PTH increases chloride conductance in distal convoluted tubule cells leading to decreased intracellular chloride activity, membrane hyperpolarization, and increased calcium entry through dihydropyridine-sensitive calcium channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.