ArticleHistochemistry and cell biology2004
A new rat type I-like alveolar epithelial cell line R3/1: bleomycin effects on caveolin expression.
Article in Histochemistry and cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- The role of alveolar epithelial junctions in early lung injury and COVID-19-induced fibrosis.Histochemistry and cell biology · 2026Article
- mRNA Levels of Epithelial and Mesenchymal Markers in Lung Epithelial Cell Lines.Reports of biochemistry & molecular biology · 2023Article
- Caveolin as a Universal Target in Dermatology.International journal of molecular sciences · 2019Review
- Article
- Potential contribution of alveolar epithelial type I cells to pulmonary fibrosis.Bioscience reports · 2017Review
- Caveolin proteins: a molecular insight into disease.Frontiers of medicine · 2016Review
- Identification of an alveolar type I epithelial cell-specific DNA nuclear import sequence for gene delivery.Gene therapy · 2016Article
- The intracellular domain of cell adhesion molecule 1 is present in emphysematous lungs and induces lung epithelial cell apoptosis.Journal of biomedical science · 2015Article
- Hyperoxia inhibits nitric oxide treatment effects in alveolar epithelial cells via effects on L-type amino acid transporter-1.Antioxidants & redox signaling · 2014Article
- Genetic ablation of caveolin-2 sensitizes mice to bleomycin-induced injury.Cell cycle (Georgetown, Tex.) · 2013Article
- Surfactant metabolism and anti-oxidative capacity in hyperoxic neonatal rat lungs: effects of keratinocyte growth factor on gene expression in vivo.Histochemistry and cell biology · 2013Article
- Assessing the relevance of in vitro studies in nanotoxicology by examining correlations between in vitro and in vivo data.Toxicology · 2012Article
- The lysophosphatidic acid receptor LPA1 promotes epithelial cell apoptosis after lung injury.American journal of respiratory cell and molecular biology · 2012Article
- RAGE and tobacco smoke: insights into modeling chronic obstructive pulmonary disease.Frontiers in physiology · 2012Article
- Validation of an LDH assay for assessing nanoparticle toxicity.Toxicology · 2011Article
- Diesel particulate matter induces receptor for advanced glycation end-products (RAGE) expression in pulmonary epithelial cells, and RAGE signaling influences NF-κB-mediated inflammation.Environmental health perspectives · 2011Article
- Purinergic P2X7 receptor regulates lung surfactant secretion in a paracrine manner.Journal of cell science · 2011Article
- Transport rather than diffusion-dependent route for nitric oxide gas activity in alveolar epithelium.Free radical biology & medicine · 2010Article
- Potential role of caveolin-1 in acetaminophen-induced hepatotoxicity.Toxicology and applied pharmacology · 2010Article
- Rac1-mediated NADPH oxidase release of O2- regulates epithelial sodium channel activity in the alveolar epithelium.American journal of physiology. Lung cellular and molecular physiology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of function and regulation of the phenotype of alveolar type I (AT I) epithelial cells is limited by the rareness of suitable cell lines or primary cultures of this cell type. We describe in the present study the type I-like rat epithelial cell line R3/1. This cell line displays in vitro a phenotype with several characteristic features of AT I cells. R3/1 cells were analysed for mRNA and protein content of markers related to the AT I cell type (T1alpha, ICAM-1, connexin-43, caveolins-1 and -2) and AT II phenotypes [surfactant proteins (SPs) A, B, C and D]. The mRNAs for SPs were found to be at a low level. Moderate protein levels for SP-A and SP-B were found, and SP-C and SP-D proteins were not detectable. R3/1 cells are positive for CD44s, E-cadherin, cytokeratin, vimentin and RAGE, and bind the lectins BPA and SBA. For demonstration of the suitability of R3/1 cells for in vitro studies on epithelial injury, the cells were treated with bleomycin. As shown by real-time RT-PCR and immunoblotting, bleomycin-treatment of R3/1 cells resulted in a decrease in mRNA and protein for both caveolin-1 and caveolin-2 in comparison with controls. The AT I-like cell line R3/1 may serve as a promising tool for the study of lung cell biology.
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