ArticlePlant cell reports2004
Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration.
Article in Plant cell reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 104 citations in OpenAlex.
- Adventitious and Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
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- Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum.Frontiers in plant science · 2022Article
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- Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots.BMC plant biology · 2016Article
- Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn).Frontiers in microbiology · 2016Article
- Composite potato plants with transgenic roots on non-transgenic shoots: a model system for studying gene silencing in roots.Plant cell reports · 2014Article
- Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb.Protoplasma · 2014Article
- The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Use of the cryptogein gene to stimulate the accumulation of Bacopa saponins in transgenic Bacopa monnieri plants.Plant cell reports · 2012Article
- Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.Plant cell reports · 2011Article
- Effect of loss of T-DNA genes on MIA biosynthetic pathway gene regulation and alkaloid accumulation in Catharanthus roseus hairy roots.Plant cell reports · 2010Article
- [Specific diagnosis of cranial sinus thrombosis].Medizinische Klinik (Munich, Germany : 1983) · 2009Article
- A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens.Physiology and molecular biology of plants : an international journal of functional plant biology · 2009Article
- Agrobacterium rhizogenes-transformed roots of coffee (Coffea arabica): conditions for long-term proliferation, and morphological and molecular characterization.Annals of botany · 2008Article
- Downregulation of terpenoid indole alkaloid biosynthetic pathway by low temperature and cloning of a AP2 type C-repeat binding factor (CBF) from Catharanthus roseus (L). G. Don.Plant cell reports · 2007Article
- Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.Plant cell reports · 2007Article
- Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures.Plant cell reports · 2007Article
- Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones.Plant cell reports · 2005Article
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5 authors at 2 institutions in 1 country.
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Abstract
Hairy root cultures of Catharanthus roseus var. Prabal were established by infecting the leaves with Agrobacterium rhizogenes agropine-type A4 strain. Two hundred and fifty independent root clones were evaluated for growth, morphology, number of integration of Ri T-DNA genes and alkaloid contents. On the basis of growth pattern, type of branching and number of lateral roots we were able to separate the hairy root clones into four categories. However based on the integration of the Ri T(L)-DNA and T(R)-DNA genes, there were only three different categories of independent hairy root clones-C1 (rolA&B(+)/ags(+)), C2 (rolA&B(-)/ags(+)) and C3 (rolA&B(+)/ags(-)). Southern hybridization analysis revealed both single and multiple copies of T-DNA integration in the root clones. The accumulation of considerable amounts of the root-specific alkaloids ajmalicine and serpentine was observed in the presence of both the T(L)-DNA and T(R)-DNA genes (C1) and the T(L)-DNA gene (C3) alone. Two rolA&B(-) but ags(+) clones (C2) accumulated much less or only very negligible amounts of ajmalicine. The possible role of the T(L)-DNA and T(R)-DNA genes on growth and alkaloid accumulation in these root clones is discussed.
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