ArticleThe Journal of cell biology1992
Clathrin heavy chain is required for pinocytosis, the presence of large vacuoles, and development in Dictyostelium.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 125 citations in OpenAlex.
- Visualization of the process of a nanocarrier-mediated gene delivery: stabilization, endocytosis and endosomal escape of genes for intracellular spreading.Journal of nanobiotechnology · 2022Article
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- Article
- Molecular characterization of clathrin heavy chain (Chc) in Rhipicephalus haemaphysaloides and its effect on vitellogenin (Vg) expression via the clathrin-mediated endocytic pathway.Experimental & applied acarology · 2020Article
- The physiological regulation of macropinocytosis duringJournal of cell science · 2018Article
- Conservation and divergence within the clathrin interactome of Trypanosoma cruzi.Scientific reports · 2016Article
- Imaging and modeling the dynamics of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium.Journal of cell science · 2012Article
- Ndm, a coiled-coil domain protein that suppresses macropinocytosis and has effects on cell migration.Molecular biology of the cell · 2012Article
- Evidence of an evolutionarily conserved LMBR1 domain-containing protein that associates with endocytic cups and plays a role in cell migration in dictyostelium discoideum.Eukaryotic cell · 2012Article
- A single β adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium.Traffic (Copenhagen, Denmark) · 2012Article
- Molecular mechanism and physiological functions of clathrin-mediated endocytosis.Nature reviews. Molecular cell biology · 2011Review
- Article
- Regulation of Hip1r by epsin controls the temporal and spatial coupling of actin filaments to clathrin-coated pits.Journal of cell science · 2010Article
- AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles.Molecular biology of the cell · 2009Article
- Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge.The Journal of cell biology · 2008Article
- Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development.Molecular biology of the cell · 2008Article
- Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium.Eukaryotic cell · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the intracellular role of the clathrin heavy chain in living cells, we have used "antisense" RNA to engineer mutant Dictyostelium discoideum cells that are severely deficient in clathrin heavy chain expression. Immunoblots stained with an anti-clathrin heavy chain antiserum revealed that mutant cells contained undetectable amounts of clathrin heavy chain protein. Similarly, Northern blots showed an absence of clathrin heavy chain mRNA. Clathrin heavy chain-deficient Dictyostelium cells were viable, but exhibited growth rates twofold slower than parental cells. Whereas many morphological features of the mutant cells were normal, mutant cells lacked coated pits and coated vesicles. Clathrin-deficient cells were also missing large translucent vacuoles that serve as endosomes and contractile vacuoles. In the absence of clathrin heavy chain, mutant cells displayed three distinct functional defects: (a) impairment in endocytosis of fluid phase markers, but competence in another endocytic pathway, the phagocytosis of solid particles; (b) defects in osmoregulation; and (c) inability to complete the starvation-induced development cycle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.