ArticleThe Journal of physiology2004
Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cells.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 85 citations in OpenAlex.
- Potential Novel Role of Membrane-Associated Carbonic Anhydrases in the Kidney.International journal of molecular sciences · 2023Review
- Blessing in disguise: when head trauma solves the riddle of carbonic anhydrase II deficiency.Radiology case reports · 2022Article
- Activation of the Ae4 (Slc4a9) cation-driven ClAmerican journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Carbonic anhydrase IX and hypoxia-inducible factor 1 attenuate cardiac dysfunction after myocardial infarction.Pflugers Archiv : European journal of physiology · 2021Article
- Acetazolamide causes renalAmerican journal of physiology. Renal physiology · 2020Article
- Review
- Carbonic anhydrase IX and acid transport in cancer.British journal of cancer · 2020Review
- Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3.American journal of physiology. Renal physiology · 2015Article
- Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.Molecular biology of the cell · 2015Article
- Analysis of the binding moiety mediating the interaction between monocarboxylate transporters and carbonic anhydrase II.The Journal of biological chemistry · 2015Article
- Regulators of Slc4 bicarbonate transporter activity.Frontiers in physiology · 2015Review
- Probing the surface of human carbonic anhydrase for clues towards the design of isoform specific inhibitors.BioMed research international · 2015Review
- Cation-coupled bicarbonate transporters.Comprehensive Physiology · 2014Review
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- NBCe1 as a model carrier for understanding the structure-function properties of Na⁺ -coupled SLC4 transporters in health and disease.Pflugers Archiv : European journal of physiology · 2014Review
- Resistance to cardiomyocyte hypertrophy in ae3-/- mice, deficient in the AE3 Cl-/HCO3- exchanger.BMC cardiovascular disorders · 2014Article
- A Case of Carbonic Anhydrase Type 2 Deficiency Syndrome with Autistic Disorder.Noro psikiyatri arsivi · 2014Article
- Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.The Journal of biological chemistry · 2014Article
- The role of membrane acid/base transporters and carbonic anhydrases for cellular pH and metabolic processes.Frontiers in neuroscience · 2014Article
- Proximal renal tubular acidosis mediated by mutations in NBCe1-A: unraveling the transporter's structure-functional properties.Frontiers in physiology · 2013Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
We have recently shown that carbonic anhydrase II (CAII) binds in vitro to the C-terminus of the electrogenic sodium bicarbonate cotransporter kNBC1 (kNBC1-ct). In the present study we determined the molecular mechanisms for the interaction between the two proteins and whether kNBC1 and CAII form a transport metabolon in vivo wherein bicarbonate is transferred from CAII directly to the cotransporter. Various residues in the C-terminus of kNBC1 were mutated and the effect of these mutations on both the magnitude of CAII binding and the function of kNBC1 expressed in mPCT cells was determined. Two clusters of acidic amino acids, L(958)DDV and D(986)NDD in the wild-type kNBC1-ct involved in CAII binding were identified. In both acidic clusters, the first aspartate residue played a more important role in CAII binding than others. A significant correlation between the magnitude of CAII binding and kNBC1-mediated flux was shown. The results indicated that CAII activity enhances flux through the cotransporter when the enzyme is bound to kNBC1. These data are the first direct evidence that a complex of an electrogenic sodium bicarbonate cotransporter with CAII functions as a transport metabolon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.