ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2004
Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1alpha.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
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- Calcium selective channel TRPV6: Structure, function, and implications in health and disease.Gene · 2022Review
- Vitamin D and Gut Health.Advances in experimental medicine and biology · 2022Article
- Villin promoter-mediated transgenic expression of transient receptor potential cation channel, subfamily V, member 6 (TRPV6) increases intestinal calcium absorption in wild-type and vitamin D receptor knockout mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Phorbol esters enhance 1α,25-dihydroxyvitamin D3-regulated 25-hydroxyvitamin D-24-hydroxylase (CYP24A1) gene expression through ERK-mediated phosphorylation of specific protein 3 (Sp3) in Caco-2 cells.Molecular and cellular endocrinology · 2012Article
- Transcriptional networks driving enhancer function in the CFTR gene.The Biochemical journal · 2012Article
- Effect of phorbol 12-myristate 13-acetate activated signaling pathways on 1α, 25 dihydroxyvitamin D3 regulated human 25-hydroxyvitamin D3 24-hydroxylase gene expression in differentiated Caco-2 cells.Journal of cellular biochemistry · 2012Article
- Genome-wide analysis of CDX2 binding in intestinal epithelial cells (Caco-2).The Journal of biological chemistry · 2010Article
- Molecular mechanisms for regulation of intestinal calcium absorption by vitamin D and other factors.Critical reviews in clinical laboratory sciences · 2010Review
- Mapping of HNF4alpha target genes in intestinal epithelial cells.BMC gastroenterology · 2009Article
- Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine.American journal of physiology. Cell physiology · 2008Article
- Inducible expression of microRNA-194 is regulated by HNF-1alpha during intestinal epithelial cell differentiation.RNA (New York, N.Y.) · 2008Article
- Intestinal resistance to 1,25 dihydroxyvitamin D in mice heterozygous for the vitamin D receptor knockout allele.Endocrinology · 2007Article
- Using genomics to understand intestinal biology.Journal of physiology and biochemistry · 2007Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Calbindin D9k (CaBP) is critical for intestinal calcium absorption; its in vivo expression is restricted to differentiated enterocytes of the small intestine. Our goal was to identify factors controlling the transcriptional regulation of this gene in the human intestine. Both the natural gene and a 4600-bp promoter construct were strongly regulated by differentiation (>100-fold) but not by treatment with 1,25(OH)2 vitamin D (<2-fold) in the Caco-2 clone TC7. Deletion-mutation studies revealed that conserved promoter sequences for cdx-2 (at -3158 bp) and hepatocyte nuclear factor (HNF)-1 (at -3131 and at -98 bp) combined to control CaBP expression during differentiation. Other putative response elements were not important for CaBP regulation in TC7 cells (CCAAT enhancer binding protein, pancreatic duodenal homebox-1 (pdx-1), a proximal cdx-2 element). Mutation of the distal HNF-1 site had the greatest impact on CaBP gene expression through disruption of HNF-1alpha binding; both basal and differentiation-mediated CaBP expression was reduced by 80%. In contrast, mutation of the distal cdx-2 element reduced only basal CaBP expression. Whereas a 60% reduction of CaBP mRNA in the duodenum of HNF-1alpha null mice confirmed the physiological importance of HNF-1alpha for CaBP gene regulation, additional studies showed that maximal CaBP expression requires the presence of both HNF-1alpha and cdx-2. Our data suggest that cdx-2 is a permissive factor that influences basal CaBP expression in enterocytes and that HNF-1alpha modulates CaBP gene expression during cellular differentiation.
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