ArticleThe Journal of membrane biology2004
Chloride channel function in the yeast TRK-potassium transporters.
Article in The Journal of membrane biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts.FEMS yeast research · 2024Review
- Dimerisation of the Yeast KInternational journal of molecular sciences · 2022Article
- Review
- Cation Transporters ofBiomolecules · 2021Review
- Review
- Evolutionary engineering inBiotechnology for biofuels · 2019Article
- A structural model for facultative anion channels in an oligomeric membrane protein: the yeast TRK (K(+)) system.Pflugers Archiv : European journal of physiology · 2015Article
- Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains.The Journal of biological chemistry · 2015Article
- Quantitative description of ion transport via plasma membrane of yeast and small cells.Frontiers in plant science · 2015Review
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- Potassium starvation in yeast: mechanisms of homeostasis revealed by mathematical modeling.PLoS computational biology · 2012Article
- Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels.Pflugers Archiv : European journal of physiology · 2011Article
- Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family.Cellular and molecular life sciences : CMLS · 2010Review
- Alkali metal cation transport and homeostasis in yeasts.Microbiology and molecular biology reviews : MMBR · 2010Review
- Diversity in expression patterns and functional properties in the rice HKT transporter family.Plant physiology · 2009Article
- Functional consequences of leucine and tyrosine mutations in the dual pore motifs of the yeast K(+) channel, Tok1p.Pflugers Archiv : European journal of physiology · 2008Article
- Chloride homeostasis in Saccharomyces cerevisiae: high affinity influx, V-ATPase-dependent sequestration, and identification of a candidate Cl- sensor.The Journal of general physiology · 2008Article
- Quantitative modeling of chloride conductance in yeast TRK potassium transporters.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
The TRK proteins-Trk1p and Trk2p- are the main agents responsible for "active" accumulation of potassium by the yeast Saccharomyces cerevisiae. In previous studies, inward currents measured through those proteins by whole-cell patch-clamping proved very unresponsive to changes of extracellular potassium concentration, although they did increase with extracellular proton concentration-qualitatively as expected for H(+) coupling to K(+) uptake. These puzzling observations have now been explored in greater detail, with the following major findings: a) the large inward TRK currents are not carried by influx of either K(+) or H(+), but rather by an efflux of chloride ions; b) with normal expression levels for Trk1p and Trk2p in potassium-replete cells, the inward TRK currents are contributed approximately half by Trk1p and half by Trk2p; but c) strain background strongly influences the absolute magnitude of these currents, which are nearly twice as large in W303-derived spheroplasts as in S288c-derived cells (same cell-size and identical recording conditions); d) incorporation of mutations that increase cell size (deletion of the Golgi calcium pump, Pmr1p) or that upregulate the TRK2 promoter, can further substantially increase the TRK currents; e) removal of intracellular chloride (e.g., replacement by sulfate or gluconate) reveals small inward currents that are K(+)-dependent and can be enhanced by K(+) starvation; and f) finally, the latter currents display two saturating kinetic components, with preliminary estimates of K(0.5) at 46 micro M [K(+)](out) and 6.8 m M [K(+)](out), and saturating fluxes of approximately 5 m M/min and approximately 10 m M/min (referred to intracellular water). These numbers are compatible with the normal K(+)-transport properties of Trk1p and Trk2p, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.