ArticleMolecular biology of the cell2004
The basolateral targeting signal of CD147 (EMMPRIN) consists of a single leucine and is not recognized by retinal pigment epithelium.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 73 citations in OpenAlex.
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- Sequence and structural insights of monoleucine-based sorting motifs contained within the cytoplasmic domains of basolateral proteins.Frontiers in cell and developmental biology · 2024Review
- Inducing human retinal pigment epithelium-like cells from somatic tissue.Stem cell reports · 2022Article
- Basigin Antibodies with Capacity for Drug Delivery Across Brain Endothelial Cells.Molecular neurobiology · 2021Article
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- Retinal pigment epithelium polarity in health and blinding diseases.Current opinion in cell biology · 2020Review
- CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells.Scientific reports · 2019Article
- A Carboxy-Terminal Monoleucine-Based Motif Participates in the Basolateral Targeting of the Na+/I- Symporter.Endocrinology · 2019Article
- Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.Hepatology (Baltimore, Md.) · 2018Article
- Alanine Scanning Mutagenesis of the C-Terminal Cytosolic End of Gpm6a Identifies Key Residues Essential for the Formation of Filopodia.Frontiers in molecular neuroscience · 2018Article
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.PloS one · 2015Article
- An EMMPRIN-γ-catenin-Nm23 complex drives ATP production and actomyosin contractility at endothelial junctions.Journal of cell science · 2014Article
- Plasma membrane protein polarity and trafficking in RPE cells: past, present and future.Experimental eye research · 2014Review
- Trafficking to the apical and basolateral membranes in polarized epithelial cells.Journal of the American Society of Nephrology : JASN · 2014Review
- Organization and execution of the epithelial polarity programme.Nature reviews. Molecular cell biology · 2014Review
- Disease-causing mutations in BEST1 gene are associated with altered sorting of bestrophin-1 protein.International journal of molecular sciences · 2013Article
- Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1.The Journal of cell biology · 2013Article
- Role of membrane traffic in the generation of epithelial cell asymmetry.Nature cell biology · 2012Review
- Expression patterns of emmprin and monocarboxylate transporter-1 in ovarian epithelial tumors.Virchows Archiv : an international journal of pathology · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
CD147, a type I integral membrane protein of the immunoglobulin superfamily, exhibits reversed polarity in retinal pigment epithelium (RPE). CD147 is apical in RPE in contrast to its basolateral localization in extraocular epithelia. This elicited our interest in understanding the basolateral sorting signals of CD147 in prototypic Madin-Darby canine kidney (MDCK) cells. The cytoplasmic domain of CD147 has basolateral sorting information but is devoid of well-characterized basolateral signals, such as tyrosine and di-leucine motifs. Hence, we carried out systematic site-directed mutagenesis to delineate basolateral targeting information in CD147. Our detailed analysis identified a single leucine (252) as the basolateral targeting motif in the cytoplasmic tail of CD147. Four amino acids (243-246) N-terminal to leucine 252 are also critical basolateral determinants of CD147, because deletion of these amino acids leads to mistargeting of CD147 to the apical membranes. We ruled out the involvement of adaptor complex 1B (AP1B) in the basolateral trafficking of CD147, because LLC-PK1 cells lacking AP1B, target CD147 basolaterally. At variance with MDCK cells, the human RPE cell line ARPE-19 does not distinguish between CD147 (WT) and CD147 with leucine 252 mutated to alanine and targets both proteins apically. Thus, our study identifies an atypical basolateral motif of CD147, which comprises a single leucine and is not recognized by RPE cells. This unusual basolateral sorting signal will be useful in unraveling the specialized sorting machinery of RPE cells.
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