ArticleAntimicrobial agents and chemotherapy2004
Extra copies of the Aspergillus fumigatus squalene epoxidase gene confer resistance to terbinafine: genetic approach to studying gene dose-dependent resistance to antifungals in A. fumigatus.
Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Role of Squalene Epoxidase Gene (Journal of fungi (Basel, Switzerland) · 2024Article
- Modular Inducible Multigene Expression System for Filamentous Fungi.Microbiology spectrum · 2022Article
- Phylogeny, Antifungal Susceptibility, and Point Mutations ofFrontiers in cellular and infection microbiology · 2022Article
- Dermatophyte Resistance to Antifungal Drugs: Mechanisms and Prospectus.Frontiers in microbiology · 2018Review
- F901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Identification and characterization of haemofungin, a novel antifungal compound that inhibits the final step of haem biosynthesis.The Journal of antimicrobial chemotherapy · 2016Article
- Molecular Characterization of Gβ-Like Protein CpcB Involved in Antifungal Drug Susceptibility and Virulence in A. fumigatus.Frontiers in microbiology · 2016Article
- Identification and characterization of a novel family of selective antifungal compounds (CANBEFs) that interfere with fungal protein synthesis.Antimicrobial agents and chemotherapy · 2015Article
- Fungal artificial chromosomes for mining of the fungal secondary metabolome.BMC genomics · 2015Article
- Invasive aspergillosis: resistance to antifungal drugs.Mycopathologia · 2012Review
- Transient and multivariate system for transformation of a fungal plant pathogen, Rosellinia necatrix, using autonomously replicating vectors.Current genetics · 2012Article
- The T788G mutation in the cyp51C gene confers voriconazole resistance in Aspergillus flavus causing aspergillosis.Antimicrobial agents and chemotherapy · 2012Article
- Ergosterol biosynthesis in Aspergillus fumigatus: its relevance as an antifungal target and role in antifungal drug resistance.Frontiers in microbiology · 2012Article
- Truncated Afyap1 attenuates antifungal susceptibility of Aspergillus fumigatus to voriconazole and confers adaptation of the fungus to oxidative stress.Mycopathologia · 2010Article
- The sho1 sensor regulates growth, morphology, and oxidant adaptation in Aspergillus fumigatus but is not essential for development of invasive pulmonary aspergillosis.Infection and immunity · 2008Article
- A Ser678Pro substitution in Fks1p confers resistance to echinocandin drugs in Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2007Article
- A Phe389Leu substitution in ergA confers terbinafine resistance in Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2006Article
- Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus.BMC microbiology · 2005Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the increasing use of antifungals such as amphotericin B, itraconazole, voriconazole, caspofungin, and terbinafine (TRB) in patients at high risk for invasive aspergillosis, resistance of Aspergillus fumigatus to these agents will ultimately emerge. Due to the limited availability of molecular genetics for A. fumigatus, few studies have addressed its mechanisms of resistance to antifungals. We transformed A. fumigatus protoplasts with a pyrG-based A. fumigatus genomic DNA library (constructed in the multicopy nonintegrating vector pRG3-AMA1-NotI, which also has the pyr-4 gene for selection). We obtained one pyrG(+) transformant that grew in medium containing a fungicidal concentration (0.625 microg/ml) of TRB. To determine whether TRB resistance in that transformant was plasmid dependent, we evicted the plasmid and found concomitant loss of uracil prototrophy and TRB resistance. DNA sequence analysis identified the gene responsible for TRB resistance as the A. fumigatus squalene epoxidase gene (ERG1), which encodes the target enzyme of TRB. Authentic A. fumigatus ERG1, amplified from the genome and cloned into pRG3-AMA1-NotI, also conferred TRB-specific resistance. This molecular approach has the potential to enhance our knowledge of the mechanisms of A. fumigatus resistance to modern antifungals.
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