ArticleJournal of biomolecular NMR2004
Novel techniques for weak alignment of proteins in solution using chemical tags coordinating lanthanide ions.
Article in Journal of biomolecular NMR, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 133 citations in OpenAlex.
- Ln-M4B3T-DOTA - a new sterically overcrowded LCT for protein pseudocontact shift NMR spectroscopy.Chemical communications (Cambridge, England) · 2026Article
- Paramagnetic Chemical Probes for Studying Biological Macromolecules.Chemical reviews · 2022Review
- A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Localization of ligands within human carbonic anhydrase II usingChemical science · 2019Article
- Conformationally locked lanthanide chelating tags for convenient pseudocontact shift protein nuclear magnetic resonance spectroscopy.Journal of biomolecular NMR · 2018Article
- Lanthanoid tagging via an unnatural amino acid for protein structure characterization.Journal of biomolecular NMR · 2017Article
- Paramagnetic Ligand Tagging To Identify Protein Binding Sites.Journal of the American Chemical Society · 2015Article
- Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy.Chemical science · 2015Article
- Visualizing transient dark states by NMR spectroscopy.Quarterly reviews of biophysics · 2015Article
- Multiple paramagnetic effects through a tagged reporter protein.Angewandte Chemie (International ed. in English) · 2015Article
- Sparse labeling of proteins: structural characterization from long range constraints.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
- Examination of matrix metalloproteinase-1 in solution: a preference for the pre-collagenolysis state.The Journal of biological chemistry · 2013Article
- How reliable are pseudocontact shifts induced in proteins and ligands by mobile paramagnetic metal tags? A modelling study.Journal of biomolecular NMR · 2013Article
- PARAssign--paramagnetic NMR assignments of protein nuclei on the basis of pseudocontact shifts.Journal of biomolecular NMR · 2013Article
- A systematic study of labelling an α-helix in a protein with a lanthanide using IDA-SH or NTA-SH tags.Journal of biomolecular NMR · 2013Article
- MaxOcc: a web portal for maximum occurrence analysis.Journal of biomolecular NMR · 2012Article
- Convenient method for resolving degeneracies due to symmetry of the magnetic susceptibility tensor and its application to pseudo contact shift-based protein-protein complex structure determination.Journal of biomolecular NMR · 2012Article
- Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR.Journal of biomolecular NMR · 2011Article
- Expanding the utility of NMR restraints with paramagnetic compounds: background and practical aspects.Progress in nuclear magnetic resonance spectroscopy · 2011Review
- Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media?Journal of biomolecular NMR · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual (15)N,(1)H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.