ArticleInfection and immunity2004
Anaplasma phagocytophilum has a functional msp2 gene that is distinct from p44.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Molecular detection and characterization ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- Molecular epidemiology, symptomatic analysis of anaplasmosis and associated study of milk reduction in cattle from district Nowshera, KP, Pakistan.Irish veterinary journal · 2025Article
- TICKHUNTER: A Targeted Hybridization-Capture Sequencing Approach for the Detection and Characterization of Tick-borne Pathogens and Blood Meals.bioRxiv : the preprint server for biology · 2025Article
- High-throughput identification of immunoreactive peptides and corresponding proteins fromFrontiers in cellular and infection microbiology · 2025Article
- Host-pathogen associations revealed by genotyping of European strains of Anaplasma phagocytophilum to describe natural endemic cycles.Parasites & vectors · 2023Article
- Insights into the mechanism regulating the differential expression of the P28-OMP outer membrane proteins in obligatory intracellular pathogenEmerging microbes & infections · 2021Article
- Co-Infection withPathogens (Basel, Switzerland) · 2021Article
- A molecular algorithm to detect and differentiate human pathogens infecting Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae).Ticks and tick-borne diseases · 2018Article
- The prevalence of Anaplasma platys and a potential novel Anaplasma species exceed that of Ehrlichia canis in asymptomatic dogs and Rhipicephalus sanguineus in Taiwan.The Journal of veterinary medical science · 2017Article
- Analysis of complete genome sequence and major surface antigens of Neorickettsia helminthoeca, causative agent of salmon poisoning disease.Microbial biotechnology · 2017Article
- Anaplasma marginale and Anaplasma phagocytophilum: Rickettsiales pathogens of veterinary and public health significance.Parasitology research · 2015Review
- Mechanisms of obligatory intracellular infection with Anaplasma phagocytophilum.Clinical microbiology reviews · 2011Review
- Anaplasma phagocytophilum infection in a domestic cat in Finland: Case report.Acta veterinaria Scandinavica · 2010Article
- Defining the immune response to Ehrlichia species using murine models.Veterinary parasitology · 2008Article
- Outer membrane protein sequence variation in lambs experimentally infected with Anaplasma phagocytophilum.Infection and immunity · 2008Article
- Identification of novel surface proteins of Anaplasma phagocytophilum by affinity purification and proteomics.Journal of bacteriology · 2007Article
- Establishment of cloned Anaplasma phagocytophilum and analysis of p44 gene conversion within an infected horse and infected SCID mice.Infection and immunity · 2005Article
- Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains.Journal of clinical microbiology · 2005Article
- Ehrlichia chaffeensis expresses macrophage- and tick cell-specific 28-kilodalton outer membrane proteins.Infection and immunity · 2005Article
- Polymorphism and transcription at the p44-1/p44-18 genomic locus in Anaplasma phagocytophilum strains from diverse geographic regions.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The msp2 and p44 genes encode polymorphic major outer membrane proteins that are considered unique to the intraerythrocytic agent of Anaplasma marginale and the intragranulocytic agent of Anaplasma phagocytophilum, respectively. In the present study, however, we found an msp2 gene in A. phagocytophilum that was remarkably conserved among A. phagocytophilum strains from human granulocytic anaplasmosis (HGA) patients, ticks, and a horse from various regions in the United States, but the gene was different in a sheep isolate from the United Kingdom. The msp2 gene in the A. phagocytophilum strain HZ genome was a single-copy gene and was located downstream of two Ehrlichia chaffeensis omp-1 homologs and a decarboxylase gene (ubiD). The msp2 gene was expressed by A. phagocytophilum in the blood from HGA patients NY36 and NY37 and by A. phagocytophilum isolates from these patients cultured in HL-60 cells at 37 degrees C. The msp2 gene was also expressed in a DBA/2 mouse infected by attaching ticks infected with strain NTN-1 and in a horse experimentally infected by attaching strain HZ-infected ticks. However, the transcript of the msp2 gene was undetectable in A. phagocytophilum strain HZ in SCID mice and Ixodes scapularis ticks infected with strain NTN-1. These results indicate that msp2 is functional in various strains of A. phagocytophilum, and relative expression ratios of msp2 to p44 vary in different infected hosts. These findings may be important in understanding roles that Msp2 proteins play in granulocytic ehrlichia infection and evolution of the polymorphic major outer membrane protein gene families in Anaplasma species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.