ArticleInfection and immunity2004
Intramolecular dimerization is required for the chlamydia-secreted protease CPAF to degrade host transcriptional factors.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- TheJournal of bacteriology · 2025Article
- Bioprospecting by Phage Display of Mimetic Peptides ofInfection and drug resistance · 2022Article
- The multiple functions of the numerousMicrobial cell (Graz, Austria) · 2019Review
- Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity.Frontiers in cellular and infection microbiology · 2018Article
- The Chlamydia-Secreted Protease CPAF Promotes Chlamydial Survival in the Mouse Lower Genital Tract.Infection and immunity · 2016Article
- Characterization of CPAF critical residues and secretion during Chlamydia trachomatis infection.Infection and immunity · 2015Article
- A path forward for the chlamydial virulence factor CPAF.Microbes and infection · 2013Review
- Article
- CPAF: a Chlamydial protease in search of an authentic substrate.PLoS pathogens · 2012Article
- Persistent Chlamydia trachomatis infection of HeLa cells mediates apoptosis resistance through a Chlamydia protease-like activity factor-independent mechanism and induces high mobility group box 1 release.Infection and immunity · 2012Article
- A Chlamydia trachomatis OmcB C-terminal fragment is released into the host cell cytoplasm and is immunogenic in humans.Infection and immunity · 2011Article
- Immunization with dendritic cells pulsed ex vivo with recombinant chlamydial protease-like activity factor induces protective immunity against genital chlamydiamuridarum challenge.Frontiers in immunology · 2011Article
- Chlamydia trachomatis secretion of proteases for manipulating host signaling pathways.Frontiers in microbiology · 2011Article
- Characterization of Pgp3, a Chlamydia trachomatis plasmid-encoded immunodominant antigen.Journal of bacteriology · 2010Article
- Autoprocessing and self-activation of the secreted protease CPAF in Chlamydia-infected cells.Microbial pathogenesis · 2010Article
- Secretion of the chlamydial virulence factor CPAF requires the Sec-dependent pathway.Microbiology (Reading, England) · 2010Article
- Role of high-mobility group box 1 protein and poly(ADP-ribose) polymerase 1 degradation in Chlamydia trachomatis-induced cytopathicity.Infection and immunity · 2010Article
- Biological characterization of Chlamydia trachomatis plasticity zone MACPF domain family protein CT153.Infection and immunity · 2010Article
- Article
- Identifying catalytic residues in CPAF, a Chlamydia-secreted protease.Archives of biochemistry and biophysics · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We previously identified a chlamydial protein designated CPAF (chlamydia protease/proteasome-like activity factor) that is secreted into host cell cytosol for degrading host transcription factors required for major histocompatibility complex antigen expression. Here we report that CPAF, synthesized as a 70-kDa proprotein, is processed into two fragments (designated CPAFn and CPAFc) to form intramolecular dimers that are much more stable than the naïve CPAF. Precipitation with antibodies that recognized CPAF dimers removed the proteolytic activity responsible for degrading host transcription factor RFX5 from chlamydia-infected host cell cytosol, while precipitation with antibodies that recognized free CPAF fragments alone did not remove this activity. Separation of CPAFn from CPAFc resulted in a loss of proteolytic activity. Furthermore, neither expressed full-length CPAF that was not processed nor coexpressed CPAFn and CPAFc fragments that failed to form dimers degraded RFX5. These observations demonstrate that intramolecular dimerization is required for CPAF to degrade host transcription factors, a strategy that is utilized by an obligate intracellular bacterial species to evade host defenses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.