ArticleInfection and immunity2004
Identification of Porphyromonas gingivalis genes specifically expressed in human gingival epithelial cells by using differential display reverse transcription-PCR.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Anti-FimA monoclonal antibodies reveal dual roles in porphyromonas gingivalis pathogenesis.Journal of oral microbiology · 2026Article
- Periodontopathogens degrade angiotensin I from the human renin-angiotensin system through surface-attached proteases.Scientific reports · 2025Article
- Evaluation of A. actinomycetemcomitans and P. gingivalis from the mouth of patients irradiated in the head and neck region: a cross-sectional study.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2024Article
- Oral Pathobiont-Derived Outer Membrane Vesicles in the Oral-Gut Axis.International journal of molecular sciences · 2024Review
- Delineation of global, absolutely essential and conditionally essential pangenomes of Porphyromonas gingivalis.Scientific reports · 2024Article
- Protease activities of vaginal Porphyromonas species disrupt coagulation and extracellular matrix in the cervicovaginal niche.NPJ biofilms and microbiomes · 2022Article
- Article
- Inherently and Conditionally Essential Protein Catabolism Genes of Porphyromonas gingivalis.Trends in microbiology · 2021Review
- Activation of vitamin D in the gingival epithelium and its role in gingival inflammation and alveolar bone loss.Journal of periodontal research · 2019Article
- Comparative analysis of expression of microbial sensing molecules in mucosal tissues with periodontal disease.Immunobiology · 2019Article
- Genes Contributing toFrontiers in cellular and infection microbiology · 2017Article
- The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.Frontiers in cellular and infection microbiology · 2017Review
- Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.BMC microbiology · 2015Article
- Genomic comparison of invasive and rare non-invasive strains reveals Porphyromonas gingivalis genetic polymorphisms.Journal of oral microbiology · 2011Article
- Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis.Infection and immunity · 2010Article
- Bacterial invasion of epithelial cells and spreading in periodontal tissue.Periodontology 2000 · 2010Review
- Response of Porphyromonas gingivalis to heme limitation in continuous culture.Journal of bacteriology · 2009Article
- Genes involved in the repression of mutacin I production in Streptococcus mutans.Microbiology (Reading, England) · 2009Article
- Role of the Clp system in stress tolerance, biofilm formation, and intracellular invasion in Porphyromonas gingivalis.Journal of bacteriology · 2008Article
- Quantitative proteomics of intracellular Porphyromonas gingivalis.Proteomics · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Porphyromonas gingivalis, one of the causative agents of adult periodontitis, can invade and survive within host epithelial cells. The molecular mechanisms by which P. gingivalis induces uptake and adapts to an intracellular environment are not fully understood. In this study, we have investigated the genetic responses of P. gingivalis internalized within human gingival epithelial cells (GECs) in order to identify factors involved in invasion and survival. We compared the differential display of arbitrarily PCR-amplified gene transcripts in P. gingivalis recovered from GECs with the display of transcripts in P. gingivalis control cultures. Over 20 potential differentially expressed transcripts were identified. Among these, pepO, encoding an endopeptidase, and genes encoding an ATP-binding cassette (ABC) transporter and a cation-transporting ATPase were upregulated in GECs. To investigate the functionality of these gene products, mutants were generated by insertional inactivation. Compared to the parental strain, mutants of each gene showed a significant reduction in their invasion capabilities. In addition, GEC cytoskeletal responses to the mutants were distinct from those induced by the parent. In contrast, adhesion of the mutant strains to GECs was not affected by lack of expression of the gene products. These results suggest that PepO, a cation-transporting ATPase, and an ABC transporter are required for the intracellular lifestyle of P. gingivalis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.