ArticleInfection and immunity2004
Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein facilitates M-cell targeting and invasion through a fibronectin bridge with host integrins.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 94 citations in OpenAlex.
- Paratuberculosis: a mucosal immunology perspective.Frontiers in immunology · 2026Review
- Encapsulin protein MAV2054 enhances Mycobacterium avium virulence by promoting Cdc42-dependent epithelial cell invasion.Journal of microbiology (Seoul, Korea) · 2025Article
- A comprehensive review of paratuberculosis in animals and its implications for public health.Open veterinary journal · 2024Review
- The role of Mce proteins in Mycobacterium avium paratuberculosis infection.Scientific reports · 2024Article
- Differential role of M cells in enteroid infection byFrontiers in cellular and infection microbiology · 2024Article
- Dectin-1-Independent Macrophage Phagocytosis ofInternational journal of molecular sciences · 2023Article
- Internalization of Leptospira interrogans via diverse endocytosis mechanisms in human macrophages and vascular endothelial cells.PLoS neglected tropical diseases · 2022Article
- Immunological Evaluation of Goats Immunized with a Commercial Vaccine against Johne's Disease.Vaccines · 2022Article
- Mycobacterial Adhesion: From Hydrophobic to Receptor-Ligand Interactions.Microorganisms · 2022Review
- The Development of 3D Bovine Intestinal Organoid Derived Models to InvestigateFrontiers in veterinary science · 2022Article
- Bacterial Species Associated With Human Inflammatory Bowel Disease and Their Pathogenic Mechanisms.Frontiers in microbiology · 2022Review
- Using Omics Approaches in the Discovery of Biomarkers for Early Diagnosis of Johne's Disease in Sheep and Goats.Animals : an open access journal from MDPI · 2021Review
- The construction ofActa pharmaceutica Sinica. B · 2020Article
- Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier.Nature reviews. Microbiology · 2018Review
- MAP1203 PromotesFrontiers in cellular and infection microbiology · 2018Article
- Article
- Mechanisms of Intestinal Epithelial Barrier Dysfunction by Adherent-InvasiveCellular and molecular gastroenterology and hepatology · 2017Review
- Aspergillus fumigatus CalA binds to integrin αNature microbiology · 2016Article
- Microfold Cells Actively Translocate Mycobacterium tuberculosis to Initiate Infection.Cell reports · 2016Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient attachment and ingestion of Mycobacterium avium subsp. paratuberculosis by cultured epithelial cells requires the expression of a fibronectin (FN) attachment protein homologue (FAP-P) which mediates FN binding by M. avium subsp. paratuberculosis. Invasion of Peyer's patches by M. avium subsp. paratuberculosis occurs through M cells, which, unlike other intestinal epithelial cells, express integrins on their luminal faces. We sought to determine if the interaction between FAP-P of M. avium subsp. paratuberculosis and soluble FN enabled targeting and invasion of M cells by M. avium subsp. paratuberculosis in vivo via these surface integrins. Wild-type and antisense FAP-P mutant M. avium subsp. paratuberculosis strains were injected alone or coinjected with blocking peptides or antibodies into murine gut loops, and immunofluorescence microscopy was performed to assess targeting and invasion of M cells by M. avium subsp. paratuberculosis. Nonopsonized M. avium subsp. paratuberculosis preferentially invaded M cells in murine gut loops. M-cell invasion was enhanced 2.6-fold when M. avium subsp. paratuberculosis was pretreated with FN. Invasion of M cells by the antisense FAP-P mutant of M. avium subsp. paratuberculosis was reduced by 77 to 90% relative to that observed for the control strains. Peptides corresponding to the RGD and synergy site integrin recognition regions of FN blocked M. avium subsp. paratuberculosis invasion of M cells by 75 and 45%, respectively, whereas the connecting segment 1 peptide was noninhibitory. Antibodies against the alpha5, alphaV, beta1, and beta3 integrin subunits inhibited M-cell invasion by 52 to 73%. The results indicate that targeting and invasion of M cells by M. avium subsp. paratuberculosis in vivo is mediated primarily by the formation of an FN bridge formed between FAP-P of M. avium subsp. paratuberculosis and integrins on M cells.
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