ArticlePharmaceutical research2004
Transfection efficiency and cytotoxicity of nonviral gene transfer reagents in human smooth muscle and endothelial cells.
Article in Pharmaceutical research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 81 citations in OpenAlex.
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- Conversion of Neural Stem Cells into Functional Neuron-Like Cells by MicroRNA-218: Differential Expression of Functionality Genes.Neurotoxicity research · 2020Article
- New-Generation Benzimidazole-Based Plasmid Delivery Reagents with High Transfection Efficiencies on the Mammalian Cells.In vitro cellular & developmental biology. Animal · 2020Article
- An improved method for increasing the efficiency of gene transfection and transduction.International journal of physiology, pathophysiology and pharmacology · 2018Article
- An efficient, non-viral dendritic vector for gene delivery in tissue engineering.Gene therapy · 2017Article
- Functionalized superparamagnetic iron oxide nanoparticles provide highly efficient iron-labeling in macrophages for magnetic resonance-based detection in vivo.Cytotherapy · 2017Article
- Dynamic architecture of the purinosome involved in human de novo purine biosynthesis.Biochemistry · 2015Article
- Hydroxyoctadecadienoic acids regulate apoptosis in human THP-1 cells in a PPARγ-dependent manner.Lipids · 2014Article
- A high content assay to assess cellular fitness.Combinatorial chemistry & high throughput screening · 2014Article
- Systemic siRNA Delivery via Peptide-Tagged Polymeric Nanoparticles, Targeting PLK1 Gene in a Mouse Xenograft Model of Colorectal Cancer.International journal of biomaterials · 2013Article
- Use of inert gas jets to measure the forces required for mechanical gene transfection.Biomedical engineering online · 2012Article
- Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons.RNA (New York, N.Y.) · 2006Article
- Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells.Genetic vaccines and therapy · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeEvaluation of a nonviral transfection reagent with respect to efficient gene transfer into primary human vascular cells.
methodsComplexes consisting of seven commercially available transfection reagents (DAC-30, DC-30, Lipofectin, LipofectAMINE PLUS, Effectene, FuGene 6 and Superfect) and EGFP encoding plasmid DNA were studied. The in vitro transfection efficiency and cytotoxicity in human aorta smooth muscle cells (HASMCs) and endothelial cells (HAECs) and rat smooth muscle cells (A-10 SMCs) were assayed in the presence of serum using flow cytometric analysis and ATP-quantitation assay, respectively.
resultsHuman primary cells were transfected less efficiently compared to the rat smooth muscle cell line. Transfection efficiency depended on the type of reagent, the reagent/DNA ratio, and, most importantly, on the cell type used. Determination of cytotoxicity showed that the effects of transfection on cell viability did not significantly differ from one another depending on the cell type. The exception to this was Superfect, which obviously reduced cell viability in all cell types.
conclusionsOur experiments showed that DAC-30 is the preferred transfection reagent for HASMCs and HAECs, exhibiting an improved efficiency combined with an acceptable cytotoxicity. Therefore, it might offer a therapeutic option for the treatment of cardiovascular disease and prove suitable for further drug development.
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