ArticleProceedings of the National Academy of Sciences of the United States of America2004
Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
89 citing papers in PubMed, 173 citations in OpenAlex.
- A tale of two distinct actin networks underlies the entire life cycle of focal adhesion.bioRxiv : the preprint server for biology · 2025Article
- Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis.Communications biology · 2025Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- Spatiotemporal analysis of F-actin polymerization with micropillar arrays reveals synchronization between adhesion sites.Molecular biology of the cell · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.bioRxiv : the preprint server for biology · 2024Article
- PCP and Septins govern the polarized organization of the actin cytoskeleton during convergent extension.Current biology : CB · 2024Article
- Imaging actin organisation and dynamics in 3D.Journal of cell science · 2024Article
- Mechanical regulation of substrate adhesion and de-adhesion drives a cell-contractile wave during Drosophila tissue morphogenesis.Developmental cell · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.Frontiers in cell and developmental biology · 2024Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.Biophysical journal · 2023Article
- On the role of myosin-induced actin depolymerization during cell migration.Molecular biology of the cell · 2023Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.bioRxiv : the preprint server for biology · 2023Article
- A network of mixed actin polarity in the leading edge of spreading cells.Communications biology · 2022Article
- An overview of substrate stiffness guided cellular response and its applications in tissue regeneration.Bioactive materials · 2022Review
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.Development (Cambridge, England) · 2022Article
- Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses.APL bioengineering · 2020Article
- Regulation of keratin network dynamics by the mechanical properties of the environment in migrating cells.Scientific reports · 2020Article
- Article
- Persistent and polarized global actin flow is essential for directionality during cell migration.Nature cell biology · 2019Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report advances in quantitative fluorescent speckle microscopy to generate simultaneous maps of cytoskeleton flow and rates of net assembly and disassembly in living cells. We apply this tool to analyze the filamentous actin (F-actin) dynamics at the front of migrating cells. F-actin turnover and flow are both known to be factors of cell locomotion. However, how they are orchestrated to produce directed cell movements is poorly understood. Our approach to data analysis allows us to examine their interdependence. Our maps confirm the previously described organization of flow into a lamellipodium and a lamellum, both exhibiting retrograde flow; and a convergence zone, where lamellum retrograde flow meets with slow anterograde flow of cortical F-actin at the ventral side of the cell body. The turnover maps show the well known actin polymerization at the leading edge, but also indicate that approximately 90% of the polymer disassembles at the lamellipodium-lamellum junction. Strong depolymerization is also found in the convergence zone, where meshwork contraction is prominent. To determine whether contraction and depolymerization are coupled events, we have treated cells with calyculin A, which is known to promote myosin activity. Stimulated contraction was accompanied by accelerated retrograde flow and increased depolymerization throughout the lamellum, whereas disassembly at the lamellipodium-lamellum junction remained unaffected. There appear to be two distinct depolymerization mechanisms, of which one depends directly on meshwork contraction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.