ArticleProceedings of the National Academy of Sciences of the United States of America2004
Biochemical and functional analysis of a 9-nt RNA sequence that affects translation efficiency in eukaryotic cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- The translational roles of circular RNAs in cancers and their underlying molecular mechanisms.Medical oncology (Northwood, London, England) · 2026Review
- Cap-Independent Circular mRNA Translation Efficiency.Vaccines · 2023Review
- Protein synthesis control in cancer: selectivity and therapeutic targeting.The EMBO journal · 2022Review
- Optimization of translation enhancing element use to increase protein expression in a vaccinia virus system.The Journal of general virology · 2021Article
- Dengue and Zika Virus 5' Untranslated Regions Harbor Internal Ribosomal Entry Site Functions.mBio · 2019Article
- Deconstructing internal ribosome entry site elements: an update of structural motifs and functional divergences.Open biology · 2018Review
- The 5' Untranslated Region of the Capsid Protein 2 Gene of Mink Enteritis Virus Is Essential for Its Expression.Journal of virology · 2018Article
- A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes.Biology methods & protocols · 2018Article
- A researcher's guide to the galaxy of IRESs.Cellular and molecular life sciences : CMLS · 2017Review
- Translational Dysregulation in Cancer: Molecular Insights and Potential Clinical Applications in Biomarker Development.Frontiers in oncology · 2017Review
- Homoiterons and expansion in ribosomal RNAs.FEBS open bio · 2015Article
- Establishment of chronic hepatitis C virus infection: translational evasion of oxidative defence.World journal of gastroenterology · 2014Review
- Article
- A mouse model to identify cooperating signaling pathways in cancer.Nature methods · 2012Article
- Review
- Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Further characterisation of the translational termination-reinitiation signal of the influenza B virus segment 7 RNA.PloS one · 2011Article
- Cellular IRES-mediated translation: the war of ITAFs in pathophysiological states.Cell cycle (Georgetown, Tex.) · 2011Review
- Facilitated leaky scanning and atypical ribosome shunting direct downstream translation initiation on the tricistronic S1 mRNA of avian reovirus.Nucleic acids research · 2010Article
- Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates.Vaccine · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We previously identified an internal ribosome entry site (IRES) within the 5' leader of the mRNA encoding the Gtx homeodomain protein and showed that shorter nonoverlapping segments of this 5' leader could enhance the translation of a second cistron in a dicistronic mRNA. One of these segments was 9 nt in length, and when multiple copies of this IRES module were linked together, IRES activity was greatly enhanced. To further expand the potential uses of these synthetic constructs and facilitate analyses of the mechanism by which they affect translation, we show here that an IRES containing five linked copies of the 9-nt sequence can also enhance translation in the 5' leader of a monocistronic mRNA. Moreover, a search for interactions of the IRES module with cellular factors revealed specific binding to 40S ribosomal subunits but not to other cellular components. Based on the results of earlier studies suggesting that this sequence could bind to a complementary segment of 18S rRNA, we tested various sequences for possible links between the length of the complementary match, their binding to ribosomes, and their influence on translational efficiency. We found that the length of the complementary match was directly correlated with the ability of RNA probes to bind to ribosomes. In addition, translation was maximally enhanced ( approximately 8-fold) by a 7-nt segment of the 9-nt element; the enhancement declined progressively as the complementary stretches became progressively longer or shorter. The results suggest that the Gtx 9-nt sequence affects translation efficiency by a mechanism that involves base pairing to 18S rRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.