ArticleProceedings of the National Academy of Sciences of the United States of America2004
A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 277 citations in OpenAlex.
- Materials and device strategies to enhance spatiotemporal resolution in bioelectronics.Nature reviews. Materials · 2025Article
- Design and synthesis of a small molecular NIR-II chemiluminescence probe for in vivoProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Pushing the limits of luminescence thermometry: probing the temperature of proteins in cells.Journal of biological physics · 2022Article
- Coupled Multiphysics Modelling of Sensors for Chemical, Biomedical, and Environmental Applications with Focus on Smart Materials and Low-Dimensional Nanostructures.Chemosensors (Basel, Switzerland) · 2022Review
- Designing Functional Bionanoconstructs for EffectiveBioconjugate chemistry · 2022Review
- Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Olfactory bulb-targeted quantum dot (QD) bioconjugate and Kv1.3 blocking peptide improve metabolic health in obese male mice.Journal of neurochemistry · 2021Article
- EM-Wave Biosensors: A Review of RF, Microwave, mm-Wave and Optical Sensing.Sensors (Basel, Switzerland) · 2019Review
- Engineering Immunological Tolerance Using Quantum Dots to Tune the Density of Self-Antigen Display.Advanced functional materials · 2017Article
- Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging.ACS applied materials & interfaces · 2017Article
- In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.Nature communications · 2016Article
- Article
- QD-Based FRET Probes at a Glance.Sensors (Basel, Switzerland) · 2015Review
- Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity.International journal of nanomedicine · 2015Review
- Protein corona - from molecular adsorption to physiological complexity.Beilstein journal of nanotechnology · 2015Review
- PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments.Beilstein journal of nanotechnology · 2014Review
- Fluorescence resonance energy transfer glucose sensor from site-specific dual labeling of glucose/galactose binding protein using ligand protection.Journal of diabetes science and technology · 2012Article
- Toward a molecular understanding of nanoparticle-protein interactions.Biophysical reviews · 2012Review
- Induction of pH sensitivity on the fluorescence lifetime of quantum dots by NIR fluorescent dyes.Journal of the American Chemical Society · 2012Article
- Quantum dot enabled molecular sensing and diagnostics.Theranostics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first generation of luminescent semiconductor quantum dot (QD)-based hybrid inorganic biomaterials and sensors is now being developed. It is crucial to understand how bioreceptors, especially proteins, interact with these inorganic nanomaterials. As a model system for study, we use Rhodamine red-labeled engineered variants of Escherichia coli maltose-binding protein (MBP) coordinated to the surface of 555-nm emitting CdSe-ZnS core-shell QDs. Fluorescence resonance energy transfer studies were performed to determine the distance from each of six unique MBP-Rhodamine red dye-acceptor locations to the center of the energy-donating QD. In a strategy analogous to a nanoscale global positioning system determination, we use the intraassembly distances determined from the fluorescence resonance energy transfer measurements, the MBP crystallographic coordinates, and a least-squares approach to determine the orientation of the MBP relative to the QD surface. Results indicate that MBP has a preferred orientation on the QD surface. The refined model is in agreement with other evidence, which indicates coordination of the protein to the QD occurs by means of its C-terminal pentahistidine tail, and the size of the QD estimated from the model is in good agreement with physical measurements of QD size. The approach detailed here may be useful in determining the orientation of proteins in other hybrid protein-nanoparticle materials. To our knowledge, this is the first structural model of a hybrid luminescent QD-protein receptor assembly elucidated by using spectroscopic measurements in conjunction with crystallographic and other data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.