ArticleMolecular biotechnology2004
The injection of plasmid DNA in mouse muscle results in lifelong persistence of DNA, gene expression, and humoral response.
Article in Molecular biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Linear DNA amplicons as a novel cancer vaccine strategy.Journal of experimental & clinical cancer research : CR · 2022Article
- COVID-19 Vaccines: Current and Future Perspectives.Vaccines · 2022Review
- SARS-CoV-2 vaccine research and development: Conventional vaccines and biomimetic nanotechnology strategies.Asian journal of pharmaceutical sciences · 2021Review
- Platforms Exploited for SARS-CoV-2 Vaccine Development.Vaccines · 2020Review
- The Race for a COVID-19 Vaccine: Current Trials, Novel Technologies, and Future Directions.Plastic and reconstructive surgery. Global open · 2020Article
- Soluble PD-1: Predictive, Prognostic, and Therapeutic Value for Cancer Immunotherapy.Frontiers in immunology · 2020Review
- Intranodal administration of mRNA encoding nucleoprotein provides cross-strain immunity against influenza in mice.Journal of translational medicine · 2019Article
- New Vaccine Technologies to Combat Outbreak Situations.Frontiers in immunology · 2018Review
- The inhibitory effects of AR/miR-190a/YB-1 negative feedback loop on prostate cancer and underlying mechanism.Scientific reports · 2015Article
- Antibiotic-free selection in biotherapeutics: now and forever.Pathogens (Basel, Switzerland) · 2015Review
- Preclinical pharmacokinetics and biodistribution of human papillomavirus DNA vaccine delivered in human endogenous retrovirus envelope-coated baculovirus vector.Pharmaceutical research · 2012Article
- A DNA vaccine against Escherichia coli O157:H7.Iranian biomedical journal · 2012Article
- Characterization of T-cell responses in macaques immunized with a single dose of HIV DNA vaccine.Journal of virology · 2010Article
- Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles.Genetic vaccines and therapy · 2008Article
- Evaluation of the persistence and gene expression of an anti-Chlamydophila psittaci DNA vaccine in turkey muscle.BMC veterinary research · 2006Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The duration of the immune response against any vaccine is critical. The present study was performed to determine the stability of injected plasmid deoxyribonucleic acid (DNA), the duration of gene expression in mouse muscle, as well as the duration of the immune response generated in mice after injection of plasmid pSO2C1 harboring the cry11Bb gene of Bacillus thuringiensis serovar. medellin. The localization and the persistence of the inoculated gene were determined by in situ hybridization and polymerase chain reaction (PCR). The results demonstrated that plasmid DNA can persist in mouse muscle for up to 2 yr. Moreover, immunohistochemical analysis showed that Cry11Bb protein was expressed for the lifetime of the mice at a low but significant level. Finally, production of Cry11Bb-specific antibodies in mice injected with pSO2C1 was high and durable as significant antibody titers were observed up to 119 wk after injection of the plasmid. This persistent immune response is likely owing to the existence of a protein and/or DNA depot in the organism, which serves to maintain the immune response, acting as a secondary or booster immunization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.