ArticleRNA (New York, N.Y.)2004
Interference probing of rRNA with snoRNPs: a novel approach for functional mapping of RNA in vivo.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- An Epstein-Barr virus-encoded snoRNA directs 2'-O-methylation of human rRNAs to control translation and the viral lytic switch.Cell reports · 2026Article
- Antisense pairing and SNORD13 structure guide RNA cytidine acetylation.RNA (New York, N.Y.) · 2022Article
- Review
- RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation.Nucleic acids research · 2021Article
- Unusual C΄/D΄ motifs enable box C/D snoRNPs to modify multiple sites in the same rRNA target region.Nucleic acids research · 2017Article
- Quality control of chemically damaged RNA.Cellular and molecular life sciences : CMLS · 2016Review
- Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation.Scientific reports · 2016Article
- Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing of a pre-mRNA Target.Acta naturae · 2012Article
- Box C/D snoRNP catalysed methylation is aided by additional pre-rRNA base-pairing.The EMBO journal · 2011Article
- The spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of the eukaryotic box C/D snoRNP nucleotide modification complex.Molecular and cellular biology · 2011Article
- Plant U13 orthologues and orphan snoRNAs identified by RNomics of RNA from Arabidopsis nucleoli.Nucleic acids research · 2010Article
- Regulation of pre-mRNA splicing in Xenopus oocytes by targeted 2'-O-methylation.RNA (New York, N.Y.) · 2010Article
- ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs.The Journal of cell biology · 2005Article
- Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451.Nucleic acids research · 2005Article
- Modified Method of rRNA Structure Analysis Reveals Novel Characteristics of Box C/D RNA Analogues.Acta naturaeArticle
- RNA under attack: cellular handling of RNA damage.Critical reviews in biochemistry and molecular biologyArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Synthesis of eukaryotic ribosomal RNAs (rRNAs) includes methylation of scores of nucleotides at the 2'-O-ribose position (Nm) by small nucleolar RNP complexes (snoRNPs). Sequence specificity is provided by the snoRNA component through base-pairing of a guide sequence with rRNA. Here, we report that methylation snoRNPs can be targeted to many new sites in yeast rRNA, by providing the snoRNA with a novel guide sequence, and that in some cases growth and translation activity are strongly impaired. Novel snoRNAs can be expressed individually or by a unique library strategy that yields guide sequences specific for a large target region. Interference effects were observed for sites in both the small and large subunits, including the reaction center region. Targeting guide RNAs to nucleotides flanking the sensitive sites caused little or no defect, indicating that methylation is responsible for the interference rather than a simple antisense effect or misguided chaperone function. To our knowledge, this is the only approach that has been used to mutagenize the backbone of rRNA in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.