ArticleThe Plant cell2004
A dominant negative mutant of cyclin-dependent kinase A reduces endoreduplication but not cell size or gene expression in maize endosperm.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 126 citations in OpenAlex.
- Baculoviruses exploit the mitotic kinase CDK1 to disrupt the nuclear lamina.PLoS pathogens · 2026Article
- Identification of novel loci associated with starch content in maize kernels by a genome-wide association study using an enlarged SNP panel.Molecular breeding : new strategies in plant improvement · 2023Article
- Riboflavin integrates cellular energetics and cell cycle to regulate maize seed development.Plant biotechnology journal · 2022Article
- Plant CDKs-Driving the Cell Cycle through Climate Change.Plants (Basel, Switzerland) · 2021Review
- Fruit size control by a zinc finger protein regulating pericarp cell size in tomato.Molecular horticulture · 2021Article
- Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.The Plant cell · 2021Article
- Nutrient-driven genome evolution revealed by comparative genomics of chrysomonad flagellates.Communications biology · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels.Plant methods · 2020Review
- Irrigation-Induced Changes in Chemical Composition and Quality of Seeds of Yellow Lupine (International journal of molecular sciences · 2019Article
- Irrigation affects characteristics of narrow-leaved lupin (Lupinus angustifolius L.) seeds.Planta · 2019Article
- The distribution pattern of endopolyploidy in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- MaizeThe Plant cell · 2019Article
- SIAMESE-RELATED1 Is Regulated Posttranslationally and Participates in Repression of Leaf Growth under Moderate Drought.Plant physiology · 2018Article
- Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino.International journal of molecular sciences · 2016Article
- Endomembrane trafficking protein SEC24A regulates cell size patterning in Arabidopsis.Plant physiology · 2014Article
- Article
- Expression, regulation and activity of a B2-type cyclin in mitotic and endoreduplicating maize endosperm.Frontiers in plant science · 2014Article
- Cell cycle control and seed development.Frontiers in plant science · 2014Review
- Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells in maize (Zea mays) endosperm undergo multiple cycles of endoreduplication, with some attaining DNA contents as high as 96C and 192C. Genome amplification begins around 10 d after pollination, coincident with cell enlargement and the onset of starch and storage protein accumulation. Although the role of endoreduplication is unclear, it is thought to provide a mechanism that increases cell size and enhances gene expression. To investigate this process, we reduced endoreduplication in transgenic maize endosperm by ectopically expressing a gene encoding a dominant negative mutant form of cyclin-dependent kinase A. This gene was regulated by the 27-kD gamma-zein promoter, which restricted synthesis of the defective enzyme to the endoreduplication rather than the mitotic phase of endosperm development. Overexpression of a wild-type cyclin-dependent kinase A increased enzyme activity but had no effect on endoreduplication. By contrast, ectopic expression of the defective enzyme lowered kinase activity and reduced by half the mean C-value and total DNA content of endosperm nuclei. The lower level of endoreduplication did not affect cell size and only slightly reduced starch and storage protein accumulation. There was little difference in the level of endosperm gene expression with high and low levels of endoreduplication, suggesting that this process may not enhance transcription of genes associated with starch and storage protein synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.