ArticleJournal of bacteriology2004
Vibrio fischeri flagellin A is essential for normal motility and for symbiotic competence during initial squid light organ colonization.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
67 citing papers in PubMed, 115 citations in OpenAlex.
- To Move or Not to Move: When and How Bacteria Suppress Flagellar Motility.Molecular microbiology · 2026Review
- Bacteria break through one-micrometer-square passages by flagellar wrapping.Nature communications · 2026Article
- Host-Associated Biofilms:Microorganisms · 2025Review
- Novel function of single-target regulator NorR involved in swarming motility and biofilm formation revealed in Vibrio alginolyticus.BMC biology · 2024Article
- Lighting the way: how theJournal of bacteriology · 2024Review
- Motility Control as a Possible Link Between Quorum Sensing to Surface Attachment in Vibrio Species.Advances in experimental medicine and biology · 2023Article
- Transitioning to confined spaces impacts bacterial swimming and escape response.Biophysical journal · 2022Article
- Functional characterization ofFrontiers in veterinary science · 2022Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- The Polar Flagellar Transcriptional Regulatory Network inJournal of bacteriology · 2021Article
- Modeled microgravity alters lipopolysaccharide and outer membrane vesicle production of the beneficial symbiont Vibrio fischeri.NPJ microgravity · 2021Article
- Outer Membrane Vesicles of Gram-Negative Bacteria: An Outlook on Biogenesis.Frontiers in microbiology · 2021Review
- Multiplexed Competition in a Synthetic Squid Light Organ Microbiome Using Barcode-Tagged Gene Deletions.mSystems · 2020Article
- Phylogenetic Distribution, Ultrastructure, and Function of Bacterial Flagellar Sheaths.Biomolecules · 2020Review
- Article
- A T3SS Regulator Mutant ofFrontiers in cellular and infection microbiology · 2020Article
- Article
- Differential expression profiling of ΔlitR and ΔrpoQ mutants reveals insight into QS regulation of motility, adhesion and biofilm formation in Aliivibrio salmonicida.BMC genomics · 2019Article
- Flagella-dependent inhibition of biofilm formation by sub-inhibitory concentration of polymyxin B in Vibrio cholerae.PloS one · 2019Article
- Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The motile bacterium Vibrio fischeri is the specific bacterial symbiont of the Hawaiian squid Euprymna scolopes. Because motility is essential for initiating colonization, we have begun to identify stage-specific motility requirements by creating flagellar mutants that have symbiotic defects. V. fischeri has six flagellin genes that are uniquely arranged in two chromosomal loci, flaABCDE and flaF. With the exception of the flaA product, the predicted gene products are more similar to each other than to flagellins of other Vibrio species. Immunoblot analysis indicated that only five of the six predicted proteins were present in purified flagella, suggesting that one protein, FlaF, is unique with respect to either its regulation or its function. We created mutations in two genes, flaA and flaC. Compared to a flaC mutant, which has wild-type flagellation, a strain having a mutation in the flaA gene has fewer flagella per cell and exhibits a 60% decrease in its rate of migration in soft agar. During induction of light organ symbiosis, colonization by the flaA mutant is impaired, and this mutant is severely outcompeted when it is presented to the animal as a mixed inoculum with the wild-type strain. Furthermore, flaA mutant cells are preferentially expelled from the animal, suggesting either that FlaA plays a role in adhesion or that normal motility is an advantage for retention within the host. Taken together, these results show that the flagellum of V. fischeri is a complex structure consisting of multiple flagellin subunits, including FlaA, which is essential both for normal flagellation and for motility, as well as for effective symbiotic colonization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.