ArticleJournal of bacteriology2004
Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Detection of antimicrobial peptides from fecal samples of FMT donors using deep learning.Frontiers in veterinary science · 2025Article
- The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges.International journal of molecular sciences · 2024Review
- Heterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp.Archives of microbiology · 2022Article
- A novel approach of recombinant laterosporulin production using the N-SH2 domain of SHP-2.BMC biotechnology · 2021Article
- Expression of five class II bacteriocins with activity againstBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Mining and Statistical Modeling of Natural and Variant Class IIa Bacteriocins Elucidate Activity and Selectivity Profiles across Species.Applied and environmental microbiology · 2020Article
- Heterologous Expression of the Class IIa Bacteriocins, Plantaricin 423 and Mundticin ST4SA, inFrontiers in microbiology · 2020Article
- Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1.PloS one · 2020Article
- Heterologous Expression of Biopreservative Bacteriocins With a View to Low Cost Production.Frontiers in microbiology · 2018Review
- Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli.Scientific reports · 2017Article
- A high-throughput expression screening platform to optimize the production of antimicrobial peptides.Microbial cell factories · 2017Article
- Antimicrobial peptide m2163 or m2386 identified from Lactobacillus casei ATCC 334 can trigger apoptosis in the human colorectal cancer cell line SW480.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Biotechnical paving of recombinant enterocin A as the candidate of anti-Listeria agent.BMC microbiology · 2014Article
- Development of a homologous expression system for and systematic site-directed mutagenesis analysis of thurincin H, a bacteriocin produced by Bacillus thuringiensis SF361.Applied and environmental microbiology · 2014Article
- Use of synthetic genes for cloning, production and functional expression of the bacteriocins enterocin A and bacteriocin E 50-52 by Pichia pastoris and Kluyveromyces lactis.Molecular biotechnology · 2014Article
- Expression of bacteriocin divercin AS7 inAnnals of microbiology · 2014Article
- Development of Class IIa Bacteriocins as Therapeutic Agents.International journal of microbiology · 2012Article
- Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe.Folia microbiologica · 2011Article
- Physiological and Structural Differences Between Enterococcus faecalis JH2-2 and Mutant Strains Resistant to (P)-Divercin RV41.Probiotics and antimicrobial proteins · 2010Article
- Bacteriocin as weapons in the marine animal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic.Marine drugs · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Divercin V41, a class IIa bacteriocin with strong antilisterial activity, is produced by Carnobacterium divergens V41. To express a recombinant version of divercin V41, we constructed a synthetic gene that encodes the mature divercin V41 peptide and then overexpressed the gene in pET-32b by using the T7 RNA polymerase promoter in the Escherichia coli Origami (DE3)(pLysS) strain. The DvnRV41 peptide was expressed as a translational fusion protein with thioredoxin and accumulated in the cell cytoplasm in a soluble anti-Listeria active form. The fusion protein was then purified and cleaved to obtain pure, soluble, folded DvnRV41 (462 microg per 20 ml of culture). This paper describes the first design of a synthetic bacteriocin gene and the first bacteriocin expressed in the E. coli cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.