Evidence map›Paper›PMID 15201341›Full record

ArticleMolecular biology of the cell2004

Links between CD147 function, glycosylation, and caveolin-1.

Wei Tang, Sharon B Chang, Martin E Hemler

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.

0numbers the graph read from it
0cells of the map it votes in
112citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

112 citing papers in PubMed, 235 citations in OpenAlex.

  1. Review
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  11. Insights into the complex interactions between Rab22a and extracellular vesicles in cancers.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2024
    Review
  12. Article
  13. Surfaceome Profiling Identifies Basigin-Chaperoned Protein Clients.Chembiochem : a European journal of chemical biology · 2023
    Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Potential protective effects of breast milk and amniotic fluid against novel coronavirus SARS-CoV-2 through decoy receptors.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2022
    Article
  19. Article
  20. Article

52 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Wei TangDana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Sharon B Chang
Martin E Hemler
Harvard University · US

Funding

NOVEL INTEGRIN-CD151-PKC SIGNALING COMPLEXR01CA086712 · NCI · DANA-FARBER CANCER INSTITUTE · PI HEMLER, MARTIN E · 2000 to 2004
$1.6M
NCI NIH HHS CA-86712NCI NIH HHS R01 CA086712
6 · The paper itself

Abstract

Cell surface CD147 shows remarkable variations in size (31-65 kDa) because of heterogeneous N-glycosylation, with the most highly glycosylated forms functioning to induce matrix metalloproteinase (MMP) production. Here we show that all three CD147 N-glycosylation sites make similar contributions to both high and low glycoforms (HG- and LG-CD147). l-Phytohemagglutinin lectin binding and swainsonine inhibition experiments indicated that HG-CD147 contains N-acetylglucosaminyltransferase V-catalyzed, beta1,6-branched, polylactosamine-type sugars, which account for its excess size. Therefore, CD147, which is itself elevated on invasive tumor cells, may make a major contribution to the abundance of beta1,6-branched polylactosamine sugars that appear on invasive tumor cells. It was shown previously that caveolin-1 associates with CD147, thus inhibiting CD147 self-aggregation and MMP induction; now we show that caveolin-1 associates with LG-CD147 and restricts the biosynthetic conversion of LG-CD147 to HG-CD147. In addition, HG-CD147 (but not LG-CD147) was preferentially captured as a multimer after treatment of cells with a homobifunctional cross-linking agent and was exclusively recognized by monoclonal antibody AAA6, a reagent that selectively recognizes self-associated CD147 and inhibits CD147-mediated MMP induction. In conclusion, we have 1) determined the biochemical basis for the unusual size variation in CD147, 2) established that CD147 is a major carrier of beta1,6-branched polylactosamine sugars on tumor cells, and 3) determined that caveolin-1 can inhibit the conversion of LG-CD147 to HG-CD147. Because it is HG-CD147 that self-aggregates and stimulates MMP induction, we now have a mechanism to explain how caveolin-1 inhibits these processes. These results help explain the previously established tumor suppressor functions of caveolin-1.

Indexed as

Amino SugarsAntigens, CDAntigens, NeoplasmBasiginCaveolin 1CaveolinsCell LineCell Line, TumorCross-Linking ReagentsEnzyme InductionGlycosylationHumansMatrix MetalloproteinasesModels, BiologicalMultiprotein ComplexesPolysaccharidesAmino SugarsAntigens, CDAntigens, NeoplasmBasiginBSG protein, humanCAV1 protein, humanCaveolin 1CaveolinsCross-Linking ReagentsMatrix MetalloproteinasesMultiprotein ComplexespolylactosaminePolysaccharidesRecombinant Fusion Proteins

Identifiers

PMID15201341
PMCPMC515339
OpenAlexW1998650485

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.