ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Ligand-dependent activation of Slo family channels is defined by interchangeable cytosolic domains.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Transmembrane determinants of voltage-gating differences between BK (Slo1) and Slo3 channels.Biophysical journal · 2024Article
- Structural mapping of patient-associated KCNMA1 gene variants.Biophysical journal · 2024Article
- SLO3 in the fast lane: The latest male contraceptive target with a promising small-molecule inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site.eLife · 2019Article
- Human sperm ion channel (dys)function: implications for fertilization.Human reproduction update · 2019Article
- A cytoplasmic Slo3 isoform is expressed in somatic tissues.Molecular biology reports · 2019Article
- Article
- Threading the biophysics of mammalian Slo1 channels onto structures of an invertebrate Slo1 channel.The Journal of general physiology · 2017Review
- A genetic variant of the sperm-specific SLO3 KThe Journal of biological chemistry · 2017Article
- Deletion of cytosolic gating ring decreases gate and voltage sensor coupling in BK channels.The Journal of general physiology · 2017Article
- Structural determinants of phosphatidylinositol 4,5-bisphosphate (PIP2) regulation of BK channel activity through the RCK1 Ca2+ coordination site.The Journal of biological chemistry · 2014Article
- Ethanol modulation of mammalian BK channels in excitable tissues: molecular targets and their possible contribution to alcohol-induced altered behavior.Frontiers in physiology · 2014Review
- Article
- Ca2+ block and flickering both contribute to the negative slope of the IV curve in BK channels.The Journal of general physiology · 2013Article
- Distinct sensitivity of slo1 channel proteins to ethanol.Molecular pharmacology · 2013Article
- Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation.Structure (London, England : 1993) · 2012Article
- Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The control of male fertility by spermatozoan ion channels.Annual review of physiology · 2012Review
- Structure and function of multiple Ca2+-binding sites in a K+ channel regulator of K+ conductance (RCK) domain.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Rediscovering sperm ion channels with the patch-clamp technique.Molecular human reproduction · 2011Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Large-conductance Ca2+- and voltage-regulated K+ channels (Slo1 BK-type) are controlled by two physiological stimuli, membrane voltage and cytosolic Ca2+. Regulation by voltage is similar to that in voltage-dependent K+ channels, arising from positively charged amino acids primarily within the S4 transmembrane helices. The basis for regulation by Ca2+ remains controversial. One viewpoint suggests that the extensive cytosolic C terminus contains the Ca2+ regulatory machinery, whereas another suggests that the pore-forming module contains the Ca2+-sensing elements. To address this issue, we take advantage of another Slo family member, the pH-regulated homolog Slo3. We reason that if the ligand-sensing apparatus is uniquely associated with a particular domain (either the pore or the cytosolic domain), exchange of those domains between Slo1 and Slo3 should result in exchange of ligand dependence in association with the key domain. The results show that the Slo3 cytosolic module confers pH-dependent regulation on the Slo1 pore module, whereas the Slo1 cytosolic module confers Ca2+-dependent regulation on the Slo3 pore module. Thus, ligand-specific regulation is defined by interchangeable cytosolic regulatory modules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.