ArticleGenes & development2004
Multiple interactions between regulatory regions are required to stabilize an active chromatin hub.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 169 citations in OpenAlex.
- Mechanisms of Globin Gene Regulation in Mammals.Annual review of genetics · 2025Review
- Enhancer Function in the 3D Genome.Genes · 2023Review
- Forced enhancer-promoter rewiring to alter gene expression in animal models.Molecular therapy. Nucleic acids · 2023Article
- Chromatin Hubs: A biological and computational outlook.Computational and structural biotechnology journal · 2022Review
- Deficiencies in the DNA Binding Protein ARID3a Alter Chromatin Structures Important for Early Human Erythropoiesis.ImmunoHorizons · 2021Article
- Transcriptional enhancers and their communication with gene promoters.Cellular and molecular life sciences : CMLS · 2021Review
- The Coup-TFII orphan nuclear receptor is an activator of the γ-globin gene.Haematologica · 2021Article
- MYC as a driver of stochastic chromatin networks: implications for the fitness of cancer cells.Nucleic acids research · 2020Article
- Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes.Nature genetics · 2019Article
- The interdependence of gene-regulatory elements and the 3D genome.The Journal of cell biology · 2019Review
- Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse.PloS one · 2019Article
- Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of Cells.Analytical chemistry · 2018Article
- Long-Range Enhancer Interactions Are Prevalent in Mouse Embryonic Stem Cells and Are Reorganized upon Pluripotent State Transition.Cell reports · 2018Article
- Enhancer functions in three dimensions: beyond the flat world perspective.F1000Research · 2018Review
- Global reorganisation ofeLife · 2017Article
- Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions.Nucleic acids research · 2016Article
- A Novel High-Content Immunofluorescence Assay as a Tool to Identify at the Single Cell Level γ-Globin Inducing Compounds.PloS one · 2015Article
- Tissue specific CTCF occupancy and boundary function at the human growth hormone locus.Nucleic acids research · 2014Article
- The hematopoietic regulator TAL1 is required for chromatin looping between the β-globin LCR and human γ-globin genes to activate transcription.Nucleic acids research · 2014Article
- Distinct chromatin configurations regulate the initiation and the maintenance of hGH gene expression.Molecular and cellular biology · 2013Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human beta-globin locus control region (LCR) is required for the maintenance of an open chromatin configuration of the locus. It interacts with the genes and the hypersensitive regions flanking the locus to form an active chromatin hub (ACH) transcribing the genes. Proper developmental control of globin genes is largely determined by gene proximal regulatory sequences. Here, we provide the first functional evidence of the role of the most active sites of the LCR and the promoter of the beta-globin gene in the maintenance of the ACH. When the human beta-globin gene promoter is deleted in the context of a full LCR, the ACH is maintained with the beta-globin gene remaining in proximity. Additional deletion of hypersensitive site HS3 or HS2 of the LCR shows that HS3, but not HS2, in combination with the beta-globin promoter is crucial for the maintenance of the ACH at the definitive stage. We conclude that multiple interactions between the LCR and the beta-globin gene are required to maintain the appropriate spatial configuration in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.