ArticleGenes & development2004
A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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110 citing papers in PubMed, 189 citations in OpenAlex.
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- Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.Nature communications · 2025Article
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- The Role of Pumilio RNA Binding Protein in Plants.Biomolecules · 2021Review
- Effects of sequence motifs in the yeast 3' untranslated region determined from massively parallel assays of random sequences.Genome biology · 2021Article
- Specific interaction of an RNA-binding protein with the 3'-UTR of its target mRNA is critical to oomycete sexual reproduction.PLoS pathogens · 2021Article
- Seeking a Role for Translational Control by Alternative Polyadenylation inMicroorganisms · 2021Review
- Puf6 primes 60S pre-ribosome nuclear export at low temperature.Nature communications · 2021Article
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- Functions of PUF Family RNA-Binding Proteins inJournal of microbiology and biotechnology · 2021Article
- Quantitative Proteomics Links the LRRC59 Interactome to mRNA Translation on the ER Membrane.Molecular & cellular proteomics : MCP · 2020Article
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- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- Regulation of Translation in the Protozoan ParasiteInternational journal of molecular sciences · 2020Review
- Functions, mechanisms and regulation of Pumilio/Puf family RNA binding proteins: a comprehensive review.Molecular biology reports · 2020Review
- Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport.eLife · 2019Article
- Functional association of Loc1 and Puf6 with RNA helicase Dhh1 in translational regulation of Saccharomyces cerevisiae Ste12.PloS one · 2019Article
50 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
Funding
Abstract
In yeast Saccharomyces cerevisiae, Ash1p, a protein determinant for mating-type switching, is segregated within the daughter cell nucleus to establish asymmetry of HO expression. The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where translation occurs. To study the mechanism regulating ASH1 mRNA translation, we isolated the ASH1 locasome and characterized the associated proteins by MALDI-TOF. One of these proteins was Puf6p, a new member of the PUF family of highly conserved RNA-binding proteins such as Pumilio in Drosophila, responsible for translational repression, usually to effect asymmetric expression. Puf6p-bound PUF consensus sequences in the 3'UTR of ASH1 mRNA and repressed the translation of ASH1 mRNA both in vivo and in vitro. In the puf6 Delta strain, asymmetric localization of both Ash1p and ASH1 mRNA were significantly reduced. We propose that Puf6p is a protein that functions in the translational control of ASH1 mRNA, and this translational inhibition is necessary before localization can proceed.
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