ArticleTransgenic research2004
Field safety assessment of recombination in transgenic grapevines expressing the coat protein gene of Grapevine fanleaf virus.
Article in Transgenic research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 108 citations in OpenAlex.
- Bridging the Gap: Genetic Insights into Graft Compatibility for Enhanced Kiwifruit Production.International journal of molecular sciences · 2025Review
- Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity againstMolecules (Basel, Switzerland) · 2022Article
- Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities.Horticulture research · 2021Review
- Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops.Plants (Basel, Switzerland) · 2021Review
- A single resistance factor to solve vineyard degeneration due to grapevine fanleaf virus.Communications biology · 2021Article
- Effect of Grafting on Viral Resistance of Non-transgenic Plum Scion Combined With Transgenic PPV-Resistant Rootstock.Frontiers in plant science · 2021Article
- Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.Transgenic research · 2018Article
- Nanobody-mediated resistance to Grapevine fanleaf virus in plants.Plant biotechnology journal · 2018Article
- Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome.Plant biotechnology journal · 2018Article
- Light intensity and temperature affect systemic spread of silencing signal in transient agroinfiltration studies.Molecular plant pathology · 2015Article
- Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock.Transgenic research · 2013Review
- Transient expression of artificial microRNAs targeting Grapevine fanleaf virus and evidence for RNA silencing in grapevine somatic embryos.Transgenic research · 2012Article
- Genetic transformation of fruit trees: current status and remaining challenges.Transgenic research · 2012Review
- "Interactive technology assessment" and beyond: the field trial of genetically modified grapevines at INRA-Colmar.PLoS biology · 2010Article
- Transgene silencing in grapevines transformed with GFLV resistance genes: analysis of variable expression of transgene, siRNAs production and cytosine methylation.Transgenic research · 2010Article
- Grapevine fanleaf virus (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana.Molecular plant pathology · 2009Article
- Assessment of the diversity and dynamics of Plum pox virus and aphid populations in transgenic European plums under Mediterranean conditions.Transgenic research · 2008Article
- Molecular characterization of grapevine plants transformed with GFLV resistance genes: I.Plant cell reports · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the major environmental safety issues over transgenic crops containing virus-derived genes relates to the outcome of recombination events between viral transgene transcripts and RNAs from indigenous virus populations. We addressed this issue by assessing the emergence of viable Grapevine fanleaf virus (GFLV) recombinants in transgenic grapevines expressing the GFLV coat protein (CP) gene. Test plants consisted of nontransgenic scions grafted onto transgenic and nontransgenic rootstocks that were exposed over 3 years to nematode-mediated GFLV infection in two distinct vineyard sites. The CP gene of challenging GFLV isolates was amplified from scions by IC-RT-PCR, and characterized by RFLP and nucleotide sequencing using strain F13 as reference since it provided the CP transgene. Analysis of EcoRI and StyI RFLP banding patterns from 347 challenging GFLV isolates and sequence data from 85 variants revealed no characteristics similar to strain F13 and no difference in the molecular variability among isolates from 190 transgenic and 157 nontransgenic plants, or from plants within (253 individuals) or outside (94 individuals) of the two sites. Interestingly, five GFLV recombinants were identified in three nontransgenic plants located outside of the two field settings. This survey indicates that transgenic grapevines did not assist the emergence of viable GFLV recombinants to detectable levels nor did they affect the molecular diversity of indigenous GFLV populations during the trial period. This is the first report on safety assessment of recombination with a transgenic crop expressing a CP gene under field conditions of heavy disease pressure but low, if any, selection pressure against recombinant viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.