Evidence map›Paper›PMID 15198198›Full record

ArticleTransgenic research2004

Analysis of Arabidopsis PsbQA gene expression in transgenic tobacco reveals differential role of its promoter and transcribed region in organ-specific and light-mediated regulation.

Tripti Gaur, Akhilesh K Tyagi

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Article in Transgenic research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tripti GaurCentre for Plant Molecular Biology, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India.
Akhilesh K Tyagi

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Arabidopsis PsbQ, encoding a 16 kDa protein of the oxygen-evolving complex, is regulated by light and is expressed preferentially in leaf tissues. To analyze the components required for light-regulated and organ-specific expression of PsbQA, several promoter constructs were generated and expressed in tobacco. The 2.2 kb promoter could confer organ-specific expression of the reporter gene, whereas regulatory elements for light-dependent induction could not be located within this promoter and the transcribed region extending up to a second exon, represented by a genomic fragment encompassing the gene. The genomic fragment representing the transcribed region, however, could confer light regulation even on a constitutive promoter, as observed by steady-state mRNA analysis in T0 and T1 tobacco plants. The results obtained have led to the conclusion that regulatory elements for organ-specificity mainly reside in the promoter region whereas the transcribed region of the gene has an important role in light regulation.

Indexed as

Gene Expression Regulation, PlantLight5' Untranslated RegionsArabidopsis ProteinsBlotting, NorthernCaulimovirusGene Expression Regulation, DevelopmentalGenes, ReporterGlucuronidaseNicotianaOrgan SpecificityPhotosynthesisPhotosystem II Protein ComplexPlants, Genetically ModifiedPromoter Regions, GeneticRegulatory Sequences, Nucleic Acid5' Untranslated RegionsArabidopsis ProteinsGlucuronidasePhotosystem II Protein ComplexpsbQ protein, ArabidopsisRNA, Messenger

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.