ArticleMolecular genetics and genomics : MGG2004
EST derived SSR markers for comparative mapping in wheat and rice.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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67 citing papers in PubMed, 192 citations in OpenAlex.
- Article
- Bioactive Compounds from Vegetal Organs ofInternational journal of molecular sciences · 2025Review
- Occurrence of simple sequence repeats in cDNA sequences of safflower (Frontiers in plant science · 2022Article
- Analysis of genetic diversity and relationships of Perilla frutescens using novel EST-SSR markers derived from transcriptome between wild-type and mutant Perilla.Molecular biology reports · 2021Article
- Genome survey of Chinese fir (Cunninghamia lanceolata): Identification of genomic SSRs and demonstration of their utility in genetic diversity analysis.Scientific reports · 2020Article
- Identification, analysis and development of salt responsive candidate gene based SSR markers in wheat.BMC plant biology · 2018Article
- Characterization and development of EST-SSR markers to study the genetic diversity and populations analysis of Jerusalem artichoke (Helianthus tuberosus L.).Genes & genomics · 2018Article
- Systemic Identification ofJournal of nucleic acids · 2017Article
- CNMS: The preferred genic markers for comparative genomic, molecular phylogenetic, functional genetic diversity and differential gene regulatory expression analyses in chickpea.Journal of biosciences · 2015Article
- Identification and analysis of novel salt responsive candidate gene based SSRs (cgSSRs) from rice (Oryza sativa L.).BMC plant biology · 2015Article
- Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.).International journal of genomics · 2015Article
- Molecular breeding in Brassica for salt tolerance: importance of microsatellite (SSR) markers for molecular breeding in Brassica.Frontiers in plant science · 2015Review
- Characterization of EST-SSR markers in durum wheat EST library and functional analysis of SSR-containing EST fragments.Molecular genetics and genomics : MGG · 2014Article
- Transcriptome sequencing and De Novo analysis of Youngia japonica using the illumina platform.PloS one · 2014Article
- A genetic linkage map of sole (Solea solea): a tool for evolutionary and comparative analyses of exploited (flat)fishes.PloS one · 2014Article
- De novo assembly and annotation of Salvia splendens transcriptome using the Illumina platform.PloS one · 2014Article
- Article
- Mapping QTL for grain yield and other agronomic traits in post-rainy sorghum [Sorghum bicolor (L.) Moench].TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Development of microsatellite markers by transcriptome sequencing in two species of Amorphophallus (Araceae).BMC genomics · 2013Article
- Pearl millet [Pennisetum glaucum (L.) R. Br.] consensus linkage map constructed using four RIL mapping populations and newly developed EST-SSRs.BMC genomics · 2013Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structural and functional relationships between the genomes of hexaploid wheat ( Triticum aestivum L.) (2n=6x=42) and rice (Oryza sativa L.) (2n=2x=24) were evaluated using linkage maps supplemented with simple sequence repeat (SSR) loci obtained from publicly available expressed sequence tags (ESTs). EST-SSR markers were developed using two main strategies to design primers for each gene: (1) primer design for multiple species based on supercluster analysis, and (2) species-specific primer design. Amplification was more consistent using the species-specific primer design for each gene. Forty-four percent of the primers designed specifically for wheat sequences were successful in amplifying DNA from both species. Existing genetic linkage maps were enhanced for the wheat and rice genomes using orthologous loci amplified with 58 EST-SSR markers obtained from both wheat and rice ESTs. The PCR-based anchor loci identified by these EST-SSR markers support previous patterns of conservation between wheat and rice genomes; however, there was a high frequency of interrupted colinearity. In addition, multiple loci amplified by these primers made the comparative analysis more difficult. Enhanced comparative maps of wheat and rice provide a useful tool for interpreting and transferring molecular, genetic, and breeding information between these two important species. These EST-SSR markers are particularly useful for constructing comparative framework maps for different species, because they amplify closely related genes to provide anchor points across species.
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