ArticleProceedings of the National Academy of Sciences of the United States of America2004
Identification of the amino acid sequence in Sindbis virus nsP4 that binds to the promoter for the synthesis of the subgenomic RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cell.PLoS computational biology · 2025Article
- Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study.International journal of molecular sciences · 2024Article
- nsP4 Is a Major Determinant of Alphavirus Replicase Activity and Template Selectivity.Journal of virology · 2021Article
- Cross-utilisation of template RNAs by alphavirus replicases.PLoS pathogens · 2020Article
- Mutation of CD2AP and SH3KBP1 Binding Motif in Alphavirus nsP3 Hypervariable Domain Results in Attenuated Virus.Viruses · 2018Article
- Alphavirus RNA synthesis and non-structural protein functions.The Journal of general virology · 2015Review
- Hypervariable domain of nonstructural protein nsP3 of Venezuelan equine encephalitis virus determines cell-specific mode of virus replication.Journal of virology · 2013Article
- Specific nucleotide changes in the subgenomic promoter region influence infectivity of the sindbis virus.Intervirology · 2013Article
- Assembly of alphavirus replication complexes from RNA and protein components in a novel trans-replication system in mammalian cells.Journal of virology · 2011Article
- Review
- Requirement for the amino-terminal domain of sindbis virus nsP4 during virus infection.Journal of virology · 2011Article
- Replicase-based plasmid DNA shows anti-tumor activity.BMC cancer · 2011Article
- hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA.Journal of biomedical science · 2010Article
- In vitro synthesis of Sindbis virus genomic and subgenomic RNAs: influence of nsP4 mutations and nucleoside triphosphate concentrations.Journal of virology · 2010Article
- Novel functions of the alphavirus nonstructural protein nsP3 C-terminal region.Journal of virology · 2010Article
- Structural and functional elements of the promoter encoded by the 5' untranslated region of the Venezuelan equine encephalitis virus genome.Journal of virology · 2009Article
- hnRNP A1 interacts with the 5' untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication.Journal of virology · 2009Article
- Article
- Properties and use of novel replication-competent vectors based on Semliki Forest virus.Virology journal · 2009Article
- Synthesis of genomic and subgenomic RNA in mosquito cells infected with two Sindbis virus nsP4 mutants: influence of intracellular nucleoside triphosphate concentrations.Journal of virology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A gel mobility-shift assay was used to demonstrate the binding of the Sindbis virus transcriptase to the promoter for the synthesis of subgenomic (SG) RNA. The assay made use of a P15 fraction (the cell fraction that is pelleted at 15,000 x g) from cells infected with recombinant vaccinia virions expressing various Sindbis virus nonstructural proteins (nsPs) and a (32)P-labeled 24-mer oligoribonucleotide representing the minimal sequence with SG promoter activity. By itself, nsP4, the viral RNA-dependent RNA polymerase, did not bind to the SG promoter; rather, all four nsPs were required for the binding of the transcriptase to the promoter. UV crosslinking of the transcriptase to a thiouridine-containing SG promoter, followed by V8 protease digestion of the complex, generated a peptide fragment that was bound to the SG promoter. This peptide fragment contained a sequence that corresponded to residues 329-334 of nsP4. This peptide may be in the fingers domain of nsP4. The peptide that was identified contained Arg residues at positions 331 and 332. Another Arg is present at position 327. By changing each of the Arg residues to Ala, we demonstrated that only the Arg residues at positions 331 and 332 were required for binding nsP4 to the SG promoter.
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