ArticleGenome research2004
An efficient method to generate chromosomal rearrangements by targeted DNA double-strand breaks in Drosophila melanogaster.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- GMMID: genetically modified mice information database.Database : the journal of biological databases and curation · 2024Article
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species.G3 (Bethesda, Md.) · 2022Article
- Cheating evolution: engineering gene drives to manipulate the fate of wild populations.Nature reviews. Genetics · 2016Review
- Review
- Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Confinement of gene drive systems to local populations: a comparative analysis.Journal of theoretical biology · 2012Article
- Warburg effect and translocation-induced genomic instability: two yeast models for cancer cells.Frontiers in oncology · 2012Article
- Different aneuploidies arise from the same bridge-induced chromosomal translocation event in Saccharomyces cerevisiae.Genetics · 2010Article
- Engineering the genomes of wild insect populations: challenges, and opportunities provided by synthetic Medea selfish genetic elements.Journal of insect physiology · 2010Review
- 53BP1 regulates DNA resection and the choice between classical and alternative end joining during class switch recombination.The Journal of experimental medicine · 2010Article
- Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.Insect molecular biology · 2009Article
- In vivo construction of transgenes in Drosophila.Genetics · 2007Article
- Article
- Highly efficient sex chromosome interchanges produced by I-CreI expression in Drosophila.Genetics · 2005Article
- Non-reciprocal chromosomal bridge-induced translocation (BIT) by targeted DNA integration in yeast.Chromosoma · 2005Article
- Genome engineering: Drosophila melanogaster and beyond.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination (HR) is an indispensable tool to modify the genome of yeast and mammals. More recently HR is also being used for gene targeting in Drosophila. Here we show that HR can be used efficiently to engineer chromosomal rearrangements such as pericentric and paracentric inversions and translocations in Drosophila. Two chromosomal double-strand breaks (DSBs), introduced by the rare-cutting I-SceI endonuclease on two different mobile elements sharing homologous sequences, are sufficient to promote rearrangements at a frequency of 1% to 4%. Such rearrangements, once generated by HR, can be reverted by Cre recombinase. However, Cre-mediated recombination efficiency drops with increasing distance between recombination sites, unlike HR. We therefore speculate that physical constraints on chromosomal movement are modulated during DSB repair, to facilitate the homology search throughout the genome.
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Registered trials
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