ArticleGenome research2004
A transcript finishing initiative for closing gaps in the human transcriptome.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Sequence and expression analysis of gaps in human chromosome 20.Nucleic acids research · 2012Article
- Recombinant human prolactin promotes human beta cell survival via inhibition of extrinsic and intrinsic apoptosis pathways.Diabetologia · 2011Article
- Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase.BMC research notes · 2010Article
- Generation and characterization of human insulin-releasing cell lines.BMC cell biology · 2009Article
- Expression analysis of matrix metalloproteinase-9 in epithelialized and nonepithelialized apical periodontitis lesions.Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics · 2009Article
- Differential patterns of receptor activator of nuclear factor kappa B ligand/osteoprotegerin expression in human periapical granulomas: possible association with progressive or stable nature of the lesions.Journal of endodontics · 2008Article
- The Schistosoma mansoni transcriptome: an update.Experimental parasitology · 2007Review
- Identification of unannotated exons of low abundance transcripts in Drosophila melanogaster and cloning of a new serine protease gene upregulated upon injury.BMC genomics · 2007Article
- DNA microarrays in herbal drug research.Evidence-based complementary and alternative medicine : eCAM · 2006Article
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Authors and funding
98 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST clusters, mapped against the genomic sequence. Each pair of EST clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. The structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. The TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.
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