ArticleMolecular biology of the cell2004
Flagellar radial spokes contain a Ca2+-stimulated nucleoside diphosphate kinase.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 95 citations in OpenAlex.
- Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025Article
- Mouse radial spoke 3 is a metabolic and regulatory hub in cilia.Nature structural & molecular biology · 2025Article
- Motile cilia genetics and cell biology: big results from little mice.Cellular and molecular life sciences : CMLS · 2021Review
- Rsph4a is essential for the triplet radial spoke head assembly of the mouse motile cilia.PLoS genetics · 2020Article
- The Role of Calmodulin in Tumor Cell Migration, Invasiveness, and Metastasis.International journal of molecular sciences · 2020Review
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- NME5 frameshift variant in Alaskan Malamutes with primary ciliary dyskinesia.PLoS genetics · 2019Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- Calaxin is required for cilia-driven determination of vertebrate laterality.Communications biology · 2019Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Calcium/calmodulin alleviates substrate inhibition in a strawberry UDP-glucosyltransferase involved in fruit anthocyanin biosynthesis.BMC plant biology · 2016Article
- Article
- Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity.The Journal of cell biology · 2014Article
- A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex.The Journal of cell biology · 2012Article
- The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes.Molecular and cellular biology · 2012Article
- Structural studies of ciliary components.Journal of molecular biology · 2012Review
- Integrated control of axonemal dynein AAA(+) motors.Journal of structural biology · 2012Review
- Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure.Cytoskeleton (Hoboken, N.J.) · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The radial spokes are required for Ca(2+)-initiated intraflagellar signaling, resulting in modulation of inner and outer arm dynein activity. However, the mechanochemical properties of this signaling pathway remain unknown. Here, we describe a novel nucleoside diphosphate kinase (NDK) from the Chlamydomonas flagellum. This protein (termed p61 or RSP23) consists of an N-terminal catalytic NDK domain followed by a repetitive region that includes three IQ motifs and a highly acidic C-terminal segment. We find that p61 is missing in axonemes derived from the mutants pf14 (lacks radial spokes) and pf24 (lacks the spoke head and several stalk components) but not in those from pf17 (lacking only the spoke head). The p61 protein can be extracted from oda1 (lacks outer dynein arms) and pf17 axonemes with 0.5 M KI, and copurifies with radial spokes in sucrose density gradients. Furthermore, p61 contains two classes of calmodulin binding site: IQ1 interacts with calmodulin-Sepharose beads in a Ca(2+)-independent manner, whereas IQ2 and IQ3 show Ca(2+)-sensitive associations. Wild-type axonemes exhibit two distinct NDKase activities, at least one of which is stimulated by Ca(2+). This Ca(2+)-responsive enzyme, which accounts for approximately 45% of total axonemal NDKase, is missing from pf14 axonemes. We found that purified radial spokes also exhibit NDKase activity. Thus, we conclude that p61 is an integral component of the radial spoke stalk that binds calmodulin and exhibits Ca(2+)-controlled NDKase activity. These observations suggest that nucleotides other than ATP may play an important role in the signal transduction pathway that underlies the regulatory mechanism defined by the radial spokes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.