ArticleMolecular biology of the cell2004
A novel intermediate in initiation complex assembly for fission yeast DNA replication.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.Nature communications · 2016Article
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.The Journal of biological chemistry · 2013Article
- Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.Genes & development · 2012Article
- Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.The EMBO journal · 2012Article
- CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.Molecular biology of the cell · 2011Article
- Auxin-inducible protein depletion system in fission yeast.BMC cell biology · 2011Article
- Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.Cell cycle (Georgetown, Tex.) · 2010Article
- How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?Genes & development · 2010Review
- The heterochromatin protein Swi6/HP1 activates replication origins at the pericentromeric region and silent mating-type locus.Nature cell biology · 2009Article
- Chromatin immunoprecipitation of replication factors moving with the replication fork.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Cell cycle regulation of DNA replication.Annual review of genetics · 2007Review
- Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.The EMBO journal · 2006Article
- Article
- Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks.The EMBO journal · 2006Article
- Current awareness on yeast.Yeast (Chichester, England) · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assembly of initiation factors on individual replication origins at onset of S phase is crucial for regulation of replication timing and repression of initiation by S-phase checkpoint control. We dissected the process of preinitiation complex formation using a point mutation in fission yeast nda4-108/mcm5 that shows tight genetic interactions with sna41(+)/cdc45(+). The mutation does not affect loading of MCM complex onto origins, but impairs Cdc45-loading, presumably because of a defect in interaction of MCM with Cdc45. In the mcm5 mutant, however, Sld3, which is required for Cdc45-loading, proficiently associates with origins. Origin-association of Sld3 without Cdc45 is also observed in the sna41/cdc45 mutant. These results suggest that Sld3-loading is independent of Cdc45-loading, which is different from those observed in budding yeast. Interestingly, returning the arrested mcm5 cells to the permissive temperature results in immediate loading of Cdc45 to the origin and resumption of DNA replication. These results suggest that the complex containing MCM and Sld3 is an intermediate for initiation of DNA replication in fission yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.