ArticleMolecular biology of the cell2004
Identification of integrin beta subunit mutations that alter heterodimer function in situ.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- DynMoCo: A novel AI framework to reveal modular substructures of protein from molecular dynamics.Biophysical journal · 2026Article
- DynMoCo: a Novel AI Framework to Reveal Modular Substructures of Protein From Molecular Dynamics.bioRxiv : the preprint server for biology · 2026Article
- The β8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development.Development (Cambridge, England) · 2022Article
- Review
- The Cytoskeletal Adapter Protein Spinophilin Regulates Invadopodia Dynamics and Tumor Cell Invasion in Glioblastoma.Molecular cancer research : MCR · 2016Article
- Protein tyrosine phosphatase-PEST and β8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.Molecular and cellular biology · 2015Article
- Article
- The effect of ligand affinity on integrins' lateral diffusion in cultured cells.European biophysics journal : EBJ · 2013Article
- Mechanical force regulates integrin turnover in Drosophila in vivo.Nature cell biology · 2012Article
- Identification of integrin beta subunit mutations that alter affinity for extracellular matrix ligand.The Journal of biological chemistry · 2011Article
- Noninvasive measurements of integrin microclustering under altered membrane cholesterol levels.Biophysical journal · 2010Article
- Tenectin is a novel alphaPS2betaPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila.Developmental biology · 2010Article
- Analysis of conserved residues in the betapat-3 cytoplasmic tail reveals important functions of integrin in multiple tissues.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Presynaptic secretion of mind-the-gap organizes the synaptic extracellular matrix-integrin interface and postsynaptic environments.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- Role of the beta-subunit arginine/lysine finger in integrin heterodimer formation and function.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Mutations in the Drosophila alphaPS2 integrin subunit uncover new features of adhesion site assembly.Developmental biology · 2007Article
- The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses.The Journal of cell biology · 2007Article
- The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18.Blood · 2007Article
- General in vivo assay for the study of integrin cell membrane receptor microclustering.Analytical chemistry · 2007Article
- Integrin structures and conformational signaling.Current opinion in cell biology · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to support the development of adult animals, including mutations in the ADMIDAS site and in a cytoplasmic NPXY motif. Many mutations in the I-like domain reduce integrin expression specifically when betaPS is combined with activating alphaPS2 cytoplasmic mutations, indicating that integrins in the extended conformation are unstable relative to the inactive, bent heterodimers. Interestingly, the screen has identified alleles that show gain-of-function characteristics in cell culture, but have negative effects on animal development or viability. This is illustrated by the allele mys(b58); available structural models suggest that the molecular lesion of mys(b58), V409>D, should promote the "open" conformation of the beta subunit I-like domain. This expectation is supported by the finding that alphaPS2betaPS (V409>D) promotes adhesion and spreading of S2 cells more effectively than does wild-type alphaPS2betaPS, even when betaPS is paired with alphaPS2 containing activating cytoplasmic mutations. Finally, comparisons with the sequence of human beta8 suggest that evolution has targeted the "mys(b58)" residue as a means of affecting integrin activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.