ArticleBiophysical journal2004
Information bounds and optimal analysis of dynamic single molecule measurements.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Subdomain dynamics enable chemical chain reactions in non-ribosomal peptide synthetases.Nature chemistry · 2024Article
- Information optimization of laser scanning microscopes for real-time feedback-driven single particle tracking.Optics express · 2023Article
- The Effect of Time Resolution on Apparent Transition Path Times Observed in Single-Molecule Studies of Biomolecules.The journal of physical chemistry. B · 2022Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling.Nature methods · 2021Article
- Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics.Biomedical optics express · 2021Article
- Information Optimal Control for Single Particle Tracking Microscopy.IFAC-PapersOnLine · 2021Article
- Prior-Apprised Unsupervised Learning of Subpixel Curvilinear Features in Low Signal/Noise Images.Biophysical journal · 2020Article
- How many photons are needed for FRET imaging?Biomedical optics express · 2020Article
- LinkingeLife · 2018Article
- Lysine Acetylation Facilitates Spontaneous DNA Dynamics in the Nucleosome.The journal of physical chemistry. B · 2015Article
- Error-based extraction of states and energy landscapes from experimental single-molecule time-series.Scientific reports · 2015Article
- Accuracy of maximum likelihood estimates of a two-state model in single-molecule FRET.The Journal of chemical physics · 2015Article
- Single-molecule dataset (SMD): a generalized storage format for raw and processed single-molecule data.BMC bioinformatics · 2015Article
- Dynamics of spontaneous flipping of a mismatched base in DNA duplex.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.Chembiochem : a European journal of chemical biology · 2013Article
- Article
- Unified resolution bounds for conventional and stochastic localization fluorescence microscopy.Physical review letters · 2012Article
- Variational Bayes analysis of a photon-based hidden Markov model for single-molecule FRET trajectories.Biophysical journal · 2012Article
- Structural distributions from single-molecule measurements as a tool for molecular mechanics.Chemical physics · 2012Article
Corrections and comments
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Time-resolved single molecule fluorescence measurements may be used to probe the conformational dynamics of biological macromolecules. The best time resolution in such techniques will only be achieved by measuring the arrival times of individual photons at the detector. A general approach to the estimation of molecular parameters based on individual photon arrival times is presented. The amount of information present in a data set is quantified by the Fisher information, thereby providing a guide to deriving the basic equations relating measurement uncertainties and time resolution. Based on these information-theoretical considerations, a data analysis algorithm is presented that details the optimal analysis of single-molecule data. This method natively accounts and corrects for background photons and cross talk, and can scale to an arbitrary number of channels. By construction, and with corroboration from computer simulations, we show that this algorithm reaches the theoretical limit, extracting the maximal information out of the data. The bias inherent in the algorithm is considered and its implications for experimental design are discussed. The ideas underlying this approach are general and are expected to be applicable to any information-limited measurement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.