ArticleBiophysical journal2004
Colloid surface chemistry critically affects multiple particle tracking measurements of biomaterials.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 267 citations in OpenAlex.
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- Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection.npj biological physics and mechanics · 2025Article
- ATP-induced reconfiguration of the micro-viscoelasticity of cardiac and skeletal myosin solutions.Applied physics letters · 2024Article
- Cell-Material Interactions in Covalent Adaptable Thioester Hydrogels.ACS biomaterials science & engineering · 2024Article
- ATP-induced cross-linking of a biomolecular condensate.Biophysical journal · 2024Article
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Characterizing rheological properties and microstructure of thioester networks during degradation.Soft matter · 2023Article
- Characterizing Phase Transitions of Microfibrillated Cellulose Induced by Anionic and Cationic Surfactants.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Micro-mechanical response and power-law exponents from the longitudinal fluctuations of F-actin solutions.Soft matter · 2023Article
- ATP-induced crosslinking of a biomolecular condensate.bioRxiv : the preprint server for biology · 2023Article
- Gelation phase diagrams of colloidal rod systems measured over a large composition space.RSC advances · 2022Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites.Frontiers in physics · 2022Article
- Interphase Chromatin Undergoes a Local Sol-Gel Transition upon Cell Differentiation.Physical review letters · 2021Article
- Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.Soft matter · 2021Article
- Imaging methods in mechanosensing: a historical perspective and visions for the future.Molecular biology of the cell · 2021Review
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Characterization of the properties of complex biomaterials using microrheological techniques has the promise of providing fundamental insights into their biomechanical functions; however, precise interpretations of such measurements are hindered by inadequate characterization of the interactions between tracers and the networks they probe. We here show that colloid surface chemistry can profoundly affect multiple particle tracking measurements of networks of fibrin, entangled F-actin solutions, and networks of cross-linked F-actin. We present a simple protocol to render the surface of colloidal probe particles protein-resistant by grafting short amine-terminated methoxy-poly(ethylene glycol) to the surface of carboxylated microspheres. We demonstrate that these poly(ethylene glycol)-coated tracers adsorb significantly less protein than particles coated with bovine serum albumin or unmodified probe particles. We establish that varying particle surface chemistry selectively tunes the sensitivity of the particles to different physical properties of their microenvironments. Specifically, particles that are weakly bound to a heterogeneous network are sensitive to changes in network stiffness, whereas protein-resistant tracers measure changes in the viscosity of the fluid and in the network microstructure. We demonstrate experimentally that two-particle microrheology analysis significantly reduces differences arising from tracer surface chemistry, indicating that modifications of network properties near the particle do not introduce large-scale heterogeneities. Our results establish that controlling colloid-protein interactions is crucial to the successful application of multiple particle tracking techniques to reconstituted protein networks, cytoplasm, and cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.