ArticleBiophysical journal2004
Detecting rearrangements of shaker and NaChBac in real-time with fluorescence spectroscopy in patch-clamped mammalian cells.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- The contribution of voltage clamp fluorometry to the understanding of channel and transporter mechanisms.The Journal of general physiology · 2019Review
- Article
- The Role of Proton Transport in Gating Current in a Voltage Gated Ion Channel, as Shown by Quantum Calculations.Sensors (Basel, Switzerland) · 2018Article
- How to Connect Cardiac Excitation to the Atomic Interactions of Ion Channels.Biophysical journal · 2018Review
- Independent movement of the voltage sensors in KScientific reports · 2017Article
- The isolated voltage sensing domain of the Shaker potassium channel forms a voltage-gated cation channel.eLife · 2016Article
- Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.Journal of molecular biology · 2015Review
- Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms.International journal of molecular sciences · 2015Article
- Patch-clamp fluorometry: electrophysiology meets fluorescence.Biophysical journal · 2014Review
- Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A limited 4 Å radial displacement of the S4-S5 linker is sufficient for internal gate closing in Kv channels.The Journal of biological chemistry · 2012Article
- Automating single subunit counting of membrane proteins in mammalian cells.The Journal of biological chemistry · 2012Article
- Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics.Journal of neuroscience methods · 2012Article
- Nanodomain Ca²⁺ of Ca²⁺ channels detected by a tethered genetically encoded Ca²⁺ sensor.Nature communications · 2012Article
- Paul F. Cranefield Award to Rikard Blunck.The Journal of general physiology · 2011Article
- State-dependent cAMP binding to functioning HCN channels studied by patch-clamp fluorometry.Biophysical journal · 2011Article
- Fluorescence techniques for determination of the membrane potentials in high throughput screening.Journal of fluorescence · 2010Review
- Rapid topology probing using fluorescence spectroscopy in planar lipid bilayer: the pore-forming mechanism of the toxin Cry1Aa of Bacillus thuringiensis.The Journal of general physiology · 2010Article
- Computational processing of optical measurements of neuronal and synaptic activity in networks.Journal of neuroscience methods · 2010Article
- Comparative study of the gating motif and C-type inactivation in prokaryotic voltage-gated sodium channels.The Journal of biological chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Time-resolved fluorescence detection of site-directed probes is a major tool in the investigation of structure-function relationships of voltage-dependent ion channels. However, the technique has been limited so far to the Xenopus-oocyte system making it difficult to study proteins, like, e.g., the prokaryotic sodium channel NaChBac, whose expression in oocytes is insufficient or whose physiological functions are distorted in oocytes. To expand the application of site-directed fluorescence detection to these proteins, we used two techniques-semiconfocal epifluorescence and total internal reflection fluorescence-to detect time-resolved fluorescence changes from site-directed labeled proteins expressed in mammalian cells under patch-clamp conditions, and investigated the characteristics and limitations of the techniques. The voltage-sensitive dye, di-8-ANEPPS, was used to monitor control of the membrane voltage in epifluorescence and total internal reflection fluorescence. Fluorescence changes in patch-clamped cells were recorded from a Shaker channel mutant (M356C) labeled in the S3-S4 linker using semiconfocal epifluorescence. The gating kinetics and fluorescence changes were in accordance with previous studies using fluorescence spectroscopy in Xenopus-oocyte systems. We applied our technique to the prokaryotic sodium channel NaChBac. Voltage-dependent protein-rearrangements of S4 could be detected that are independent of inactivation. Comparison of the S3-S4 linker regions revealed structural differences to the KvAP voltage sensor. The results from the NaChBac channel point to structural requirements for the S3-S4 loop to generate a fluorescence signal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.